摘要
目的了解广州地区婴幼儿扎如病毒性腹泻感染现状。方法在2008年8月—2009年7月,每周二、周五采集10份腹泻标本,运用real-time RT-PCR进行检测,阳性标本再行RT-PCR扩增、纯化、克隆、测序。结果扎如病毒检出率为1.0%(10/985),包括GI与GIV型,其中GI型占90%(9/10)。结论扎如病毒是该地区婴幼儿急性腹泻中检出率较低的病原体,GI-1是主要的感染型,GIV型感染在华南地区有增多趋势。
Objective To investigate the prevalence of sapoviral infection in Guangzhou. Methods The stool samples and the corresponding case information were collected in infants and children with acute diarrhea during August 2008 to July 2009. The sapoviras in stools was screened by the real-time fluorescence quantitative PCR, and then the positive samples were further amplified, purified, and cloned for sequencing. Results The positive rate of Sapovims in stools was 1.0% （10/985） including the genotypes of GIV and GI, in which genotype of GI accounted for 90% （9/ 10）. Conclusions There is a lower detection rate of sapovimses which is a pathogen causing acute diarrhea in the infants and children in Guangzhou. The genotype of GI-1 is the major type of infection, and the prevalence of GIV genotype tends to increase in the South China.
出处
《临床儿科杂志》
CAS
CSCD
北大核心
2011年第6期514-517,共4页
Journal of Clinical Pediatrics
关键词
扎如病毒
基因型
腹泻
儿童
sapovirus
genotype
diarrhea
children
