摘要
目的对OLYMPUS AU5421和Beckman CX9两种不同检测系统的多个项目进行方法比对和偏倚评估,探讨同一医院不同检测系统间测定结果是否具有可比性。方法依据美国国家临床实验室标准委员会EP9-A2文件要求,每天收集8份不同浓度的新鲜患者血清,分别用两种检测系统测定钾、钠、氯(Cl-)、葡萄糖、尿素氮及肌酐等6项生化指标,连续测定5 d,记录测定结果,计算相关系数和直线回归方程,根据回归方程计算各指标在医学决定水平上的预期偏倚和预期偏倚的95%可信区间并判断偏倚是否可以接受。结果 Cl-的预期偏倚的95%可信区间大于CLIA′88允许误差范围的1/4,不可接受,其余5个项目测定结果的预期偏倚均可以接受。结论不同检测系统应定期对相同测定指标进行方法比对和偏倚评估,以确保检测结果的可比性。
Objective To explore the comparability of multiple projects detected by OLYMPUS AU5421 Beckman CX9 detection systems in the same hospital by methodology comparing and bias assessing. Methods Ba on the U. S. National Committee for Clinical Laboratory Standards （NCCLS） EPg-A2,8 copies of different eoncent tions of fresh serum were collected,and then we tested 6 biochemical projects of K＋ ,Na＋ ,C1-,Glu,Bun,and Cr with two detection systems for 5 days, then we recorded the results and calculated correlation coefficient and linear regr sion equation. We calculated each indicator in the medical decision level of the expected bias and the expected bias the 95% confidence interval and determined the bias according to the regression equation. Results The expected bias of the 95 % confidence interval of C1- was larger than 1/4 of CLIA′88 permissible error range,and it was unacceptable. The expected bias of the remaining 5 projects could be accepted. Conclusion Methodology comparison and bias estimation of the same test items in different detection systems should be carried out regularly in order to cns comparability of test results.
出处
《检验医学与临床》
CAS
2011年第10期1203-1205,共3页
Laboratory Medicine and Clinic
