摘要
目的探讨不同化学发光检测系统测定人生长激素(hGH)水平的可比性。方法对15例做人生长激素(hGH)激发试验的患儿采集75份静脉血标本(基础水平、激发试验后30、60、90、120min),使用3种化学发光系统:化学发光酶免疫分析系统(CLEIA)、电化学发光免疫分析系统(ECLIA)和微粒子化学发光免疫分析系统(CMIA),同时测定血清hGH水平,并计算各时间点hGH与其基础水平的比值(hGH-R),进一步使用Bland-Altman法对方法学间的一致性进行分析。结果(1)CLEIAECLIA间只有基础水平、ECLIA-CMIA间于激发试验后120min hGH水平具有较好一致性。其余任意2个检测系统间、于任何时间点hGH水平均不一致。(2)引入hGH-R后,只在ECLIA-CMIA间、于激发试验后30min具有较好的一致性,其余各时间点、任意2个检测系统间均不一致。结论血清hGH水平仅偶尔在特定检测系统间、于激发试验特定时间点具有一致性;hGHR的引入,对提高hGH在各检测系统间的可比性帮助不大。
Objective To investigate the comparability of human growth hormone（hGH）levels by different chemiluminescence detection system.Methods Totally 75 venous blood samples（including samples at baseline,30,60,90,120 mins after the GHTT）were collected from 15 children with the growth hormone stimulation test.Serum hGH levels were measured by three chemiluminescence systems（CLEIA,ECLIA and CMIA）,HGH-R at each time point was calculated,and Bland-Altman method was used to analyze the consistency between the two methods.Results（1）CLEIA-ECLIA was only basal level,120 mins after CLEIA-CMIA stimulation,hGH level had a good consistency.Between the other two detection systems,hGH levels were not consistent at any time point.（2）After hGH-R was introduced,ECLIA-CMIA,30 mins after stimulation,had a good consistency.The remaining time points,any two detection systems were not consistent.Conclusion Serum hGH levels are only occasionally consistent at specific detection system,in the excitation test specific time point.The introduction of hGH-R does not help to improve the comparability of hGH among the detection systems.
出处
《检验医学与临床》
CAS
2017年第2期161-163,166,共4页
Laboratory Medicine and Clinic
基金
卫生部医药卫生科技发展研究中心专项课题资助项目(28-5-5)
首都临床特色应用研究(吴阶平)(Z1411020060000)
关键词
人生长激素
生长激素激发试验
化学发光免疫分析系统
human growth hormone
growth hormone provocation test
chemiluminescent immunoassay systems
