摘要
目的:研究玻璃体腔注入热休克蛋白27(HSP27)对大鼠慢性青光眼模型血清中HSP27抗体浓度的影响。方法:51只Wistar大鼠随机分为高眼压组(15只),假手术对照组(15只),致敏组(9只),致敏对照组(9只),正常组(3只),右眼为实验眼,左眼为对照眼。术前、术后第3天、1周、2周、4周、6周、8周均检测大鼠双眼眼压。按时间点依次处死大鼠,抽血供酶联免疫吸附(ELISA)法检测HSP27抗体含量。结果:①高眼压组、致敏组和致敏对照组右眼术后眼压明显升高(p<0.01);②高眼压组和致敏对照组术后血清中HSP27抗体浓度明显升高(P<0.05),致敏组术后血清HSP27抗体浓度未见升高(p>0.05)。结论:①随着眼压升高和持续时间延长,大鼠血清中的HSP27抗体水平逐渐升高;②玻璃体腔内注射HSP27能完全抑制高眼压状态下血清中HSP27抗体水平的升高。
Objective： To investigate the function of HSP27 in glaucoma optic nerve disease and whether it can be prevented or cured by some way.Methods：51 Wistar rats are divided into the ocular hypertension group（15）,sham control group（15）,immunized group（9）,immunized control group（9） and normal group（3） at random,all the right eyes are experimental eyes,all the left eyes are control eyes.IOP of the rats was measured before operation,at the 3rd day,the 1st week,the 2nd week,the 4th week,the 6th week,the 8th week after operation.The rats were killed in time and blood serum is gathered for ELISA to measure the level of HSP27 antibody.Results：①IOP of right eye is elevated obviously in ocular hypertension group、immunized group and immunized control group（p0.01）.②HSP27 antibody in blood serum level is elevated obviously after operation in ocular hypertension group and immunized control group（p0.05）.HSP27 antibody in blood serum level is not elevated after operation in immunized group（p0.05）.Conclusions： ①Along with intraocular hypertension and elevated IOP in the duration of the extension,HSP27 antibody in blood serum levels increased gradually.②Injecting HSP27 into vitreous cavity can completely inhibit elevated HSP27 antibody in blood serum level against ocular hypertension greatly.
出处
《川北医学院学报》
CAS
2011年第3期207-211,共5页
Journal of North Sichuan Medical College
基金
国家自然科学基金项目(30660196)
