摘要
目的探讨硝苯地平(NF)对人肝星状细胞系(HSC)-LX2细胞的活化及分泌转化生长因子(TGF)-β的影响。方法体外建立干扰素(IFN)-γ刺激HSC活化的培养体系,设3组:对照组、IFN-γ组和IFN-γ+NF组,对照组仅加入培养基,IFN-γ组加入IFN-γ和培养基,IFN-γ+NF组加入IFN-γ、NF和培养基,于0、1、2、3、5 d观察HSC-LX2活化增殖和分泌细胞因子的能力,采用细胞爬片和免疫组化技术鉴定活化HSC的细胞形态,流式细胞术分析HSC表达α-平滑肌肌动蛋白(α-SMA)水平,ELISA法测定不同组HSC分泌TGF-β的浓度。结果与对照组比较,IFN-γ组HSC活化和分泌TGF-β的能力较强,差异有统计学意义(P<0.05);与IFN-γ组比较,IFN-γ+NF组HSC活化和分泌TGF-β的能力较弱,差异有统计学意义(P<0.05)。结论 NF体外实验中能抑制HSC-LX2的增殖和活化,具有潜在的抗纤维化作用。
Objective To investigate the effect of nifedipine （NF） on the regulation of proliferation and function of human hepatic stellate cell（HSC） lines, and to illuminate its further pharmacological role. Methods Activation of HSC culture system stimulated by IFN-γ was established in vitro, which was separated three groups：control, IFN-γand IFN-γ＋ NF. The control group was added medium only. The IFN-γ group was added medium and IFN-γ. The IFN-γ ＋ NF group was added medium, IFN-γ and NF. Slide of crawling cell was adopted to identify morphology of HSC cell. Flow cytometer was employed to detect the activation of HSC by analyzing the level of α-SMA. ELISA was used to determine the concentration of TGF-β. Results Compared with the control group, IFN-γ could induce the expression of α-SMA and TGF-β of HSC in vitro（P 〈0. 05）. However, NF could inhibit the expression of α- SMA and TGF-β of HSC （ P 〈 0. 05 ）. Conclusion NF can inhibit the proliferation and activation of HSC which suppress the occurrence of liver fibrosis.
出处
《安徽医科大学学报》
CAS
北大核心
2011年第6期542-545,共4页
Acta Universitatis Medicinalis Anhui
关键词
硝苯地平
肝硬化
转化生长因子Β
nifedipine
liver cirrhosis
transforming growth factor beta
