摘要
目的:通过建立大鼠肺缺血再灌注模型,观察N-乙酰半胱氨酸(NAC)对大鼠缺血再灌注损伤肺的保护作用,并探讨其作用机制。方法:随机将30只雌性SD大鼠分为3组(每组10只):假手术组(A组),左侧开胸后切断下肺韧带,于肺门处套绕阻断带,维持通气165 min;I/R组(B组),阻断左肺门45 min,开放再通气2 h;NAC组(C组),于麻醉前30 min腹腔注射NAC,余操作同B组。实验结束时放血处死动物,检测各组肺湿-干重比(W/D),髓过氧化物酶(MPO)、超氧化物歧化酶(SOD)的活性,丙二醛(MDA)的含量,血清TNF-α浓度和肺组织学改变。结果:(1)肺组织损伤A组轻微,B组严重,C组损伤程度重于A组,轻于B组。(2)B、C组造模后肺组织W/D、MPO活性及MDA含量均较A组升高(P<0.05),SOD活性较A组降低(P<0.05),C组的肺组织W/D、MPO活性及MDA含量较B组降低(P<0.05),SOD活性较B组升高(P<0.05)。(3)再灌注2 h后C组TNF-α含量较B组降低(P<0.05)。结论:NAC对在体SD大鼠肺缺血再灌注组织有一定的保护作用,这与其清除氧自由基,抑制中性粒细胞的浸润及激活,抑制TNF-α的产生有关。
Objective： To observe the protective effect of N-acetylcysteine（NAC） on lung ischemia-reperfusion injury in rats by establishing models of lung ischemia-reperfusion.Methods： Thirty female SD rats were randomly divided into three groups of ten each： sham group（group A）,left chest was opened and main bronchus and pulmonary artery and vein were isolated but not clamped.The lungs were ventilated for 165 min.Ischemia-reperfusion group（group B）,left pulmonary hilum was isolated and clamped for 45 min.After declamping,the lungs were ventilated for 2 h.NAC group（group C）,were injected NAC（200 mg·kg-1） intraperitoneally 30 min before anesthesia.All rats were killed at the end of the reperfusion.The blood were collected for the measurement of TNF-α.The left lung tissue were excised for microscopic examination and measurement of wet/dry weight（W/D） ratio,myeloperoxidase（MPO） activity,superoxide dismutase（SOD） activity and malondialdehyde（MDA） content.Results：（1）There were remarkable pathological manifestations of pulmonary injury in the group B.Compared with the group B,the pathomorphology changes of group C were ameliorated.（2） In group B,C,the W/D ratio,content of MDA and activity of MPO in lung tissue after reperfusion were significantly higher than those of group A（P0.05）,the activity of SOD in lung tissue after reperfusion were significantly lower than that of group A（P0.05）.Compared with group B,the W/D ratio,the levels of MDA and the activity of MPO were significantly decreased in group C（P0.05）,the levels of SOD were significantly increased in group C（P0.05）.（3）In group C,the content of TNF-α in blood after reperfusion were significantly lower than that of group B（P0.05）.Conclusion： Injected NAC intraperitoneally before anesthesia protects the lungs against ischemia-reperfusion injury in SD rats in vivo.This protection is associated with the inhibition of infiltration and activation of neutrophils and the reduction of TNF-α.It is also associated with the cleaning of oxygen free radicals.
出处
《东南大学学报（医学版）》
CAS
2011年第3期440-444,共5页
Journal of Southeast University(Medical Science Edition)
基金
江苏省卫生厅重大科研项目(H200542)
