摘要
目的 为缺血疗法在胰腺癌的临床应用提供基础研究资料。方法 将人胰腺癌细胞株ＰＣ－ ３ 移植于裸小鼠胰腺右叶，获得小鼠胰腺移植癌，并结扎荷癌裸鼠胃十二指肠动脉、胰十二指肠下动脉及背胰动脉诱导胰腺右叶区域性缺血，观察缺血对移植癌增殖动力学及病理形态学的影响。结果 缺血组移植癌生长缓慢，倍增时间延长，移植癌体积、癌细胞增殖指数及蛋白质含量术后３、７、１４天均显著低于对照组（Ｐ＜ ０．０１）。光镜下缺血组移植癌呈大片凝固坏死，并可见渐进性坏死细胞、炎症细胞浸润及纤维组织增生；癌周胰腺组织腺泡萎缩，出现纤维化及淋巴细胞浸润。结论 缺血对裸小鼠胰腺移植癌具有确切的杀伤和抑制作用，该模型的建立为临床中晚期胰腺癌的缺血治疗提供了一定的理论基础及实验依据。
Objective To provide experimental data for the clinical application of ischemia therapy in treating pancreatic cancer.Methods The pancreatic transplanted cancer was obtained by transplanting human pancreatic cancer cell line PC-3 into right lobe of the pancreas in nude mice.Regional ischemia of right lobe of the pancreas was induced with ligating gastroduodenal,inferior pancreaticoduodenal and dorsal pancreatic arteries of the cancer-bearing animals.Effects of ischemia on proliferative kinetics and pathomorphology of the pancreatic transplanted cancer of nude mice were investigated.Results The transplanted cancer of ischemia group grew slower and their doubling time was longer as compared to the control.On the 3rd,7th and 14th days after operation,the volume of transplanted cancer,the proliferative index and protein content of the cancer cells in ischemia group were significently lower than those of control(P<0.01).Optical microscopy revealed large areas of coagulative necrosis,necrobiotic cells,inflammatory miflammatory cells proliferation and fibrosis infiltration were seen in ischemia group.The atrophy of acini,fibrosis and the infiltration of lyphocytes were found in peripancreatic tissue.Conclusions Ischemia can destroy and inhibit the pancreatic transplanted cancer of nude mice effectively.Establishment of the animal model has provided experimental data for the clinical application of ischemia therapy in treating pancreatic cancer.
出处
《肝胆外科杂志》
1999年第5期389-391,共3页
Journal of Hepatobiliary Surgery
关键词
缺血疗法
胰腺肿瘤
动物模型
Ischemia therapy Pancreatic neoplasm Animal model
