摘要
目的通过研究胰腺癌Bcl-2、Bax和血管内皮生长因子((VEGF)的变化情况与细胞凋亡的关系,探讨康莱特和5-Fu治疗胰腺癌的机制。方法建立人胰腺癌PC-3细胞裸鼠皮下移植瘤模型,采用免疫组织化学方法检测Bcl-2、Bax和VEGF在胰腺癌移植瘤中的表达,流式细胞仪检测细胞凋亡比率,测量肿瘤体积。结果康莱特和5-Fu都可明显提高胰腺癌细胞凋亡率,降低细胞Bcl-2在蛋白水平上的表达,两者对Bax在蛋白水平上的表达无影响;康莱特明显降低移植瘤VEGF蛋白的表达;康莱特和5-Fu联合组,胰腺癌细胞凋亡比率显著高于单药组,而Bcl-2蛋白表达率显著低于单药组;康莱特和联合用药组VEGF蛋白表达率低于5-Fu组;联合用药组肿瘤体积小于单药组。结论康莱特联合5-Fu治疗胰腺癌的效果明显优于两药单独使用的效果。
Objective To investigate the mechanism of Kanglaite combined with 5-Fu in treating pancreatic carcinoma through studying the changes of Bcl-2, Bax and vascular endothelial growth factor（VEGF） in pancreatic carcinoma cell and the relationship between apoptosis and these changes. Methods The model of athymic mouse subcutaneous transplantation tumor of human pancreatic carcinoma PC-3 cell was established; the expression of Bcl- 2, Bax and VEGF were detected in malignant cells using immunohistochemistry; the apoptosis proportion was detected by adopting flow cytometry; the tumor size was detected. Results Kanglaite combined with 5-Fu could obviously depress the expression of Bcl-2 in protein level in malignant cells; apoptosis proportion had no effect on Bax expression; Kanglaite could obviously depress the expression of VEGF in malignant cells. The apoptosis proportion was higher and protein expression of Bcl-2 was obviously lower in group of Kanglaite combined with 5-Fu than that in Kanglaite group or 5-Fu group. What was more, the protein expression of VEGF and the tumor volum was lowest in group of Kanglaite combined with 5-Fu. Conclusion The effect of Kanglaite united with 5-Fu on treatment of pancreatic cancer is better than that of Kanglaite or 5-Fu alone.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2005年第5期473-476,共4页
Journal of Xi’an Jiaotong University（Medical Sciences）
