摘要
采用荧光和UV光谱法研究了盐酸药根碱(Jat)与牛血清白蛋白(BSA)相互作用的光谱特性。结果表明:Jat对于BSA荧光猝灭主要是静态猝灭和非辐射能量转移;Jat浓度增大,BSA荧光峰被猝灭的同时出现峰裂分现象,原来345 nm处的单峰逐渐裂分为二重峰,其中一峰峰值蓝移,另一峰峰值红移至λem=362～365 nm;测得不同温度下的结合常数及结合位点数;热力学常数探讨作用机理,主要以疏水作用力与BSA相互作用;作用过程是一个熵增加、G ibbs自由能降低的自发超分子作用过程。同步荧光技术研究了Jat对BSA构象的影响,表明BSA荧光主要源于色氨酸残基,Jat对BSA的构象均有影响。F rster偶极-偶极非辐射能量转移理论,计算了Jat与BSA作用距离<7 nm。
The interactions between jatrorrhizine（Jat） and bovine serum albumin（BSA） were studied by fluorescence and ultraviolet-visible absorption spectra.The binding constants and binging sites were measured at different temperatures.The results revealed that Jat have strong ability to quench the intrinsic fluorescence of BSA.The phenomenon that fluorescence spectra of BSA splitted and shifted from a simple peak to double peaks in the presence of Jat concentration accretion.Fluorescence quenching was thought to be deduced by combining static with diffusion collision moving quenching and nonradiative energy transfer.Based on thermodynamic parameters,the acting forces were determined to be hydrophobic force.Based on the mechanism of the Frster energy transference,the transfer efficiency of energy E and transfer distance r between acceptor Jat and donor BSA were obtained.
出处
《化学试剂》
CAS
CSCD
北大核心
2011年第6期486-488,496,共4页
Chemical Reagents
基金
国家自然科学基金资助项目(20875059)
山西省归国留学基金资助项目(2008)
山西省高校科技研究开发项目(20091036)
