摘要
以β-环糊精和蜜二糖为原料,通过α-半乳糖苷酶酶法合成得到半乳糖基β--环糊精(Gal-β-CD)。酶法合成条件为:蜜二糖中酶用量为20U/g,β-环糊精和蜜二糖的物质的量比为1:2,pH6.5(50mmol/L醋酸缓冲液),反应时间24h,反应温度40℃。经过高效液相色谱分离产物,通过质谱、红外光谱和核磁共振证明该产物为6-O-α-D-半乳糖基-β-环糊精。
Galactosyl-β-cyclodextrin（Gal-β-CD） was synthesized under the catalysis of α-D-galactosidase derived from germinated coffee beans using melibiose as a donor and β-cyclodextrin as an acceptor.The optimum synthesis parameters were enzyme dose 20 U/g melibiose,β-CD-to-melibiose molar ratio 1：2,pH 6.5（50 mmol/L acetate buffer）,reaction time 24 h and reaction temperature 40 ℃,respectively.The product obtained under the optimized conditions was purified by preparative HPLC,and the purified product was 6-O-α-D-galactosyl-β-cyclodextrin according to ESI-MS,IR,and NMR analyses.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第12期57-59,共3页
Food Science
基金
武汉工业学院引进人才科研启动项目(2010RZ20)
武汉工业学院校立科研计划项目(2009Q01)
