摘要
目的 研究１ ，２５ 二羟维生素 Ｄ３［１ ，２５（ Ｏ Ｈ）２ Ｄ３］ 对乳腺癌细胞株 Ｍ Ｃ Ｆ７ 生长及凋亡的影响。 方法 采用四唑氮蓝比色（ Ｍ Ｔ Ｔ） 法检测细胞增殖，光镜和电镜形态学观察，流式细胞仪测定细胞周期和凋亡率，末端脱氧核苷酸转移酶介导的原位酶标记（ Ｔ Ｕ Ｎ Ｅ Ｌ） 法计数凋亡细胞，免疫印迹法检测ｂｃｌ２ 蛋白表达。 结果 １０ － ７ ｍｏｌ／ Ｌ 的１ ，２５（ Ｏ Ｈ）２ Ｄ３ 就可以抑制 Ｍ Ｃ Ｆ７ 增生，改变细胞周期时相分布，致 Ｇ０／ Ｇ１ 期阻滞，加强细胞毒性化疗药阿霉素（ Ａｄｒ） 的作用，促进细胞凋亡，下调ｂｃｌ２ 蛋白表达。 结论 １ ，２５（ Ｏ Ｈ）２ Ｄ３ 可以作为细胞调亡诱导剂成为新一类乳腺癌激素治疗药。
Objective To study the effect of 1,25 dihydroxyvitamin D 3 [1,25(OH) 2D 3] on growth and apoptosis in breast cancer cell line MCF 7. Methods We compared cell numbers by using MTT method, analyzed cell cycle and apoptosis percentage by flow cytometric, observed cellular structure and ultrastructure, determinaed quantitatively apoptosis cells by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL), and detected expression of bcl 2 protein by Western Blot. Results Incubation with 1,25(OH) 2D 3 10 -7 mol/L, MCF 7 cells exhibited significant growth inhibition. Flow cytometric analysis indicated cell′s G 0/G 1 arrest along with increasing apoptotic peak and percentage. The action of cytotoxic drug Adr was strengthened. Western Blot showed that 1,25(OH) 2D 3 could downregulate bcl 2 protein expression. Conclusions 1,25(OH) 2D 3 could be a new hormone for the treatment of breast cancer.
出处
《中华外科杂志》
CAS
CSCD
北大核心
1999年第8期497-499,共3页
Chinese Journal of Surgery
关键词
肿瘤细胞
乳腺癌
MCF-7
二羟维生素D3
Breast neoplasms Cholecalciferol Growth inhibitors Tumor cells,cultured
