摘要
在用双酶消化法组建 DNA 物理图谱的过程中,我们总结了一套"三三规则"即:三类片段(消失片段 Fd,保留片段 Fr,接头片段 Fl);三种片段关系(Fd_1=Fd_2,Fdi=1/2 Fl,Fl_1=Fl_2),三种排列(Fl 的首尾排列,Fr 的夹心排列,Fd 的相嵌排列).用这一套规则组建了大尺蠖核型多角体病毒4个限制内切酶的物理图谱。其片段顺序分别是:BamHI,HCIDEBGFA;Xhol,AJBCEIHFDG;HindⅢ,AFBCMHLJKGIED;BglⅡ,BEAG-DFC.
The insect baculovius BsNPV DNA was mapped with double endonuclease digestions.Using BamHI-H frament containing BsNPV polyhedrin gene serve as zero site,the order of the four restrition fragments are determined as follows:BglⅡ,BEAGDFC;BamHI,HCIDEBGFA;HindⅢ,AFBCMHLJ- KGIED;XhoⅠ,AJBCEIHFDG.Here we present a three three rule for restriction mapping of DNA based on our experiences and practices.They are:three type fragments,e.g,disappearance fragment (Fd),retained frag- ment(Fr) and liker fragment(Fl);three type aligments;e.g,head tail for Fl,sandwich for Fr,and mosaic for Fd;three relations among fragments, e.g,Fd_1=Fd_2,Fd_i=1/2ΣFl_i;Fl_1=Fl_2.This rule seems to have simplified the method and procedure for the construction of DNA restriction map.
出处
《武汉大学学报（自然科学版）》
CSCD
1990年第2期89-96,共8页
Journal of Wuhan University(Natural Science Edition)
基金
国家科学基金
国家教委资助的课题
关键词
昆虫
病毒
DNA
物理图谱
双酶消化
Inseet baculovirus
DNA restriction map
Double digestions
