摘要
为开发微生物源的天然色素作为食品着色剂,对产紫罗兰蓝色素的粤蓝链霉菌在不同培养基中的产色水平进行了测试,对接种量、培养温度、摇床转速、发酵时间4个参数进行了正交优化.利用硅胶柱层析、葡聚糖凝胶柱层析、薄层层析、高效液相色谱等方法分离纯化色素成分,利用核磁共振、质谱等方法测定结构,发现该菌在高氏合成一号培养基中色素产量最高,该培养基是合适的工业化生产基础培养基.优化的培养条件为:接种量5%,培养温度30℃,180r/min振动培养7天.从色素粗提物中纯化出7种单体化合物,分别为榴菌素、榴菌素B、醌茜兰类色素Zg、Zgg、二氢Zg及MM44785和去鼠李糖基MM44785,其中二氢Zg和去鼠李糖基MM44785是2种新化合物.
In order to develop natural pigments from microbes as food colorants,the production level of Streptomyces vietnamensis for violet-blue pigment was tested in different media,and such four parameters as the inoculation quantity,the culture temperature,the rotation speed and the fermentation time were orthogonally optimized.Then,the constituents of the pigment were isolated and purified by means of the silica gel column chromatography,the sephadex gel filtration chromatography,the thin-layer chromatography and the high-performance liquid chromatography,and the structure of the pigment was identified by means of the nuclear magnetic resonance and the mass spectrometry.The results show that Gauze＇s synthetic No.1 medium is suitable for the industrial production of violet-blue pigment due to its highest productivity,and that the optimal fermentation conditions are as follows： an inocu-lation quantity of 5%,a culture temperature of 30 ℃,a rotation speed of 180 r/min and a culture time of 7 d.Moreover,seven compounds,namely granaticin,granaticin B,MM44785,L-rhodinoside-deglycosylated MM44785 as well as quinizarin-derived pigments Zg,Zgg and dihydro-Zg,can be isolated and purified from the crude extract,among which dihydro-Zg and L-rhodinoside-deglycosylated MM44785 both possess novel structures.
出处
《华南理工大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2011年第5期132-137,共6页
Journal of South China University of Technology(Natural Science Edition)
基金
广东省科技计划项目(2008B021400005)
广州市科技计划项目(2008J1-C091)
广东省科学院科技创新引导项目(cx200702)
关键词
粤蓝链霉菌
天然色素
纯化
鉴定
结构
榴菌素
Streptomyces vietnamensis
natural pigment
purification
identification
structure
granaticin
