摘要
为研究日本血吸虫单克隆抗独特型抗体 Ｎ Ｐ３０ 对血吸虫病肝纤维化的影响，将４５ 只 Ｉ Ｃ Ｒ 小鼠随机分为两组，实验组腹腔注射 Ｎ Ｐ３０，对照组腹腔注射 Ｓ Ｐ２／０ 腹水。尾蚴攻击感染后第４、８、１２、１６、２０、２４ 周分别处死小鼠取肝， Ｖ Ｇ 组化染色和Ⅰ、Ⅲ型胶原、纤维连接蛋白（ Ｆ Ｎ）免疫组化染色，应用 Ｎ Ｙ Ｄ１０００ 型计算机图像分析系统对肝纤维化程度进行定量测定。实验结果表明， Ｖ Ｇ 染色显示实验组肝组织胶原的平均光密度值、卵均面积和总面积明显小于对照组。免疫组化显示实验组Ⅰ、Ⅲ型胶原及 Ｆ Ｎ 含量比对照组低。本文提示 Ｎ Ｐ３０ 对血吸虫病肝纤维化有明显的抑制作用，是一很有希望的血吸虫病抗病疫苗候选分子。
In order to observe the effect of the monoclonal anti idiotypic antibody NP30 of Schistosoma japonicum on hepatic fibrosis of schistosomiasis. The experimental group of ICR mice was actively immunized by injecting NP30 intraperitoneally and the control group was given an injection of SP2/0 ascites intraperitoneally. The mice were killed at the 4th, 8th, 12th, 16th, 20th, 24th week, respectively. Livers were removed and stained with histochemistry of Van Gieson (VG) and immunohistochemistry of collagen type Ⅰ,Ⅲ and fibronectin (FN). The content of hepatic fibrosis was determined quantitatively by NYD 1000 Image Analysis System. As a result, the VG stain revealed that the average optical density, area per egg and total area of hepatic collagen in the experimental group were lower obviously than those in the control group. The immunohistochemical stain indicated that the content of collagen type Ⅰ, Ⅲ and FN in the former were lower than that in the latter. The results above indicated that NP30 could act as an effective inhibitor to hepatic fibrosis of schistosomiasis and a potential candidate of anti pathologic vaccine molecule against schistosomiasis.
出处
《中国寄生虫病防治杂志》
CSCD
1999年第3期222-223,共2页
Chinese Journal of Parasitic Disease Control
基金
国家自然科学基金
关键词
日本血吸虫
抗独特型抗体
免疫组化
血吸虫病
Schistosoma japonicum
anti idiotypic antibody
hepatic fibrosis
immunohistochemistry
image analysis
