摘要
分别采用溶剂浸提和水蒸汽蒸馏法对白苏叶进行提取,并用生长速率法分别测定2种白苏叶提取物对7种植物病原真菌的抑制作用。测定结果表明,在0.1 g干样/mL下,白苏叶乙醇提取物在3~5 d的培养时间内能够完全抑制辣椒疫霉病菌的生长,对番茄灰霉病菌的生长抑制率高于60%。白苏叶挥发油在测定中对植物病原真菌也表现出较强的抑菌活性。白苏叶挥发油在0.5 mg/mL时能够完全抑制供试7种供试病菌的生长。白苏挥发油对7种供试植物病原真菌的测定表明,挥发油对水稻纹枯病菌和番茄灰霉病菌具有较强的抑制作用,EC50分别为0.0677mg/mL和0.1050 mg/mL。
Cold-soak extracting and steam distillation methods were applied to extract antifungal components from Perilla frutescens leaves.The antifungal activities of ethanol extracts and essential oil were tested against seven plant pathogens using growth rate method.Results showed that ethanol extracts and essential oil of leaves had strong antifungal activities.At the concentration of 0.1 g D.W./mL,the ethanol extracts inhibited the growth of mycelia of Phytophthora capsici completely,and the inhibition rates against Botrytis cinnerea were higher than 60%.The essential oil of Perilla frutescens leaves had strong inhibition action on tested fungi.At the concentration of 0.5 mg/mL,the essential oil inhibited the growth of mycelia of seven pathogens completely.Inhibition toxicity test showed that essential oil had better inhibition action against Thanatephorus cucumeris and Botrytis cinnerea,and the EC50 were 0.0677 mg/mL and 0.1050 mg/mL,respectively.
出处
《北京农学院学报》
2011年第2期18-20,共3页
Journal of Beijing University of Agriculture
基金
河南省高校中青年骨干教师资助项目
河南科技学院高层次人才科研项目(09013)
河南省重点科技攻关项目(082102350006)
2009年大学生创新实验项目(2009CXSY32)
关键词
白苏
乙醇提取物
挥发油
抑菌活性
Perilla frutescens
ethanol extract
essential oil
antifungal activity
