摘要
在基础饲料中分别添加0%、0.2%、0.3%和0.4%艾佐迈(azomite),饲养平均体质量为(64.20±1.68)g的草鱼(Ctenopharyngodon idella)8周,研究其对草鱼生长性能、肠道消化酶活性和血清非特异性免疫的影响.结果表明:饲料中添加艾佐迈可在不同程度上改善草鱼生长性能,其中添加0.2%艾佐迈使增质量率提高16.65%(P=0.08),饲料系数降低12.90%(P〈0.05);添加0.2%和0.4%艾佐迈可显著提高草鱼的肥满度(P〈0.05);艾佐迈对草鱼肌肉水分、灰分、粗脂肪和粗蛋白含量均无影响(P〉0.05);添加0.2%-0.4%艾佐迈可在不同程度上提高草鱼前肠蛋白酶、淀粉酶和脂肪酶活性及血清超氧化物歧化酶(superoxide dismutase,SOD)和碱性磷酸酶(alkaline phosphatase,AKP)活性,其中0.2%艾佐迈添加组可显著提高血清SOD活性(P〈0.05);饲料中添加0.2%、0.3%艾佐迈对草鱼肝胰脏谷草转氨酶(glutamic-oxalacetic transaminase,GOT)和谷丙转氨酶(glutamic-pyruvic transaminase,GPT)活性无影响(P〉0.05),但添加0.4%艾佐迈组显著降低了肝胰脏GOT、GPT活性.上述结果表明:饲料中添加艾佐迈可提高草鱼的生长性能和血清非特异性免疫力;草鱼饲料中艾佐迈添加量建议为0.2%.
An experiment was conducted to investigate the effect of azomite supplementation on growth performance,intestinal digestive enzyme activities and serum nonspecific immune of grass carp(Ctenopharyngodon idella).Four diets adding respectively 0%(control group),0.2%,0.3%,0.4% azomite were fed to grass carp with initial body mass of(64.20±1.68) g for 8 weeks.The results showed that growth performance of grass carp was increased in different degrees by supplemental azomite in diets,in which mass gain rate was increased by 16.65%(P=0.08) and feed conversion rate(FCR) was decreased by 12.90%(P〈0.05) by supplementation of 0.2% azomite in diet,compared with control group.Condition factors(CF) of fish fed with diet adding 0.2%,0.4% azomite were significantly higher than control fish(P〈0.05).There was no significant difference in the contents of muscle moisture,ash,crude fat and crude protein among treatments(P〈0.05).Activities of protease,lipase,amylase in intestine and superoxide dismutase(SOD),alkaline phosphatase(AKP) in serum of fish were enhanced in different degrees(P〈0.05) by addition of 0.2%-0.4% azomite in diet.Fish fed with diet adding 0.2% azomite had a significantly higher SOD activity than control fish(P〈0.05).Hepatopancreas glutamic-oxalacetic transaminase(GOT) and glutamic-pyruvic transaminase(GPT) activities were not affected by 0.2%,0.3% azomite addition,but significantly decreased by 0.4% azomite addition.The results above show that the addition of azomite can improve the growth performance and serum nonspecific immune function.The proper dose of azomite in grass carp diet is suggested to be 0.2%.
出处
《浙江大学学报(农业与生命科学版)》
CAS
CSCD
北大核心
2011年第3期312-318,共7页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
上海市重点学科建设资助项目(Y1101)
上海市科委重大基础专项资助项目(06DJ14003)
关键词
草鱼
艾佐迈
生长性能
消化酶
非特异性免疫
Ctenopharyngodon idella
azomite
growth performance
digestive enzyme
nonspecific immunity