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改良蛋白印迹法在基因产物生物学行为研究的应用 被引量:10

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摘要 本文通过对蛋白印迹法(western blotting)酶联生色底物的改进,形成一套完善、系统的操作规程,并用于其基因产物学行为的研究。经重复性、平行性试验证明:改良蛋白印迹法具有使酶联生色反应的放大效应更加灵敏、快捷的特点,具有特异性强、检出限低、样品蛋白容易获取等优点。应用改良蛋白印迹法检测20例大肠癌组织及其非瘤粘膜组织PRAD-1/CCND1基因产物CyclinD1蛋白(p36^D1)。
出处 《中国实验临床免疫学杂志》 1999年第4期1-5,共5页
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