摘要
目的探讨胞质分裂阻滞微核（CBMN）分析在估算辐射事故受照射者的生物剂量中的应用价值。方法2008年山西太原辐射事故发生后16h收集5名受照射者（1、2、3、4和5号）的外周血及1号的骨髓，进行CBMN分析，以微核（MN）频率估算生物剂量。对较严重的受照射者（1号）结合体外。^60Co γ射线大剂量照射实验获得的核分裂指数（NDI）估算剂量。照射后1年内对5名受照射者的微核频率进行动态分析。结果1号患者的微核频率超过了微核剂量一效应曲线上限所对应的数值，用CBMN法结合NDl分析（CBMN＋NDI）对其估算的剂量范围为10～20Gy。以微核频率对2、3、4和5号的剂量估算分别为3．6、2．9、2，3和2．9Gy一法结果与染色体畸变分析及物理模拟估算的剂量接近，与临床表现基本相符。1号照射后26d（移植后18d）微核频率大幅度下降（u=3.295，P〈0.05）。2～5号照射后各时间点所分析的微核频率逐渐减少，照射后1个月时微核频率与照射后16h相比除2号外，差异均有统计学意义（u：6．874、4．526及7.811，P〈0.05）。结论CBMN法快速、准确，与染色体畸变分析结合使用可相互补充和验证。新指标CBMN＋NDI对于高剂量范围的估计具有参考价值。
Objective To investigate the value of cytokinesis-block micronuclei （CBMN） assay in estimation of the biological doses of the victims of radiation accident. Methods Samples of peripheral blood were collected from the 5 victims （Subjects 1-5 ） at 16 h after the radiation accident of Taiyuan, Shanxi Province. And the peripheral blood samples and bone marrow sample were collected from the victim No. 1 at 23 and 24 h after the radiation. Eight days after the accident Subject 1 underwent allogeneic peripheral blood hematopoietic stem cell transplantation. At different time points in the period of 1 year after the accident, peripheral blood samples were collected from these 5 victims. CBMN assay was conducted on the peripheral blood lymphocytes on the samples, and the biological doses were estimated based on tile micronuclei （MN） frequencies. The nuclear division index （NDI） obtained from in vitro irradiation experiment using high dose of ^60Co γ-rays was used to estimate the exposed doses for Subject 1. Dynamic analysis of the MN frequency for the 5 victims was performed in the period of 1 year after the accident. Results The MN frequency of Subject 1 surpassed the value corresponding to the upper limit of the MN dose-effective curve. The dose range estimated by the combination of the CBMN and NDI （ CBMN ＋ NDI） assay was 10-20 Gy for Subject 1. The doses estimated by MN frequency for Subjects 2, 3, 4, and 5 were 3.6, 2.9, 2.3, and 2.9 Gy, respectively. The estimated doses were in accordance with those estimated by physical method, chromosome aberration analysis, and clinical symptoms. Prominent decrease of the MN frequency was observed at 26 d after the accident （18 d after the transplantation） for Subject 1 （ u = 3.295, P 〈 0.05 ）. Gradual decrease of MN frequency was observed after the accident for Subjects 2, 3, 4, and 5. The MN frequencies 1 month after the accident of Subjects 3, 4, and 5 were all significantly lower than those 16 h later （u = 6.874, 4.526, and 7.811, P 〈 0.05）. Conclusions Quick and accurate, CBMN assay reinforces and verifies the result of chromosome aberration analysis. The new index CBMN ＋ NDI assay is of reference value for estimating higher dose of irradiation.
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
2011年第2期185-189,共5页
Chinese Journal of Radiological Medicine and Protection
关键词
辐射事故
微核
生物剂量估算
Radiation accident
Micronuclei
Biodosimetry
