摘要
目的:探讨活化的p38MAPK在苦参碱(Matrine,Mat)诱导人Burkitt′s淋巴瘤Raji细胞凋亡过程中对Fas/FasL的影响。方法:选用Raji细胞进行体外培养,用苦参碱(终浓度为0.4、0.8、1.6 mg/mL)和在此基础上加入SB203580(p38 MAPK抑制剂)作用48 h后,分别采用AnnexinⅤ-FITC/PI双染法检测细胞凋亡率和Western blot检测P-p38MAPK、Fas、FasL蛋白表达的变化。结果:终浓度为0.4、0.8、1.6 mg/mL苦参碱对Raji细胞总凋亡率显著高于SB203580组及对照组(P<0.05或P<0.01);Western blot显示P-p38MAPK、Fas、FasL蛋白表达水平随苦参碱浓度的提高而增加,并且在加入SB203580后降低。P-p38MAPK与Fas、FasL具有明显相关性。结论:苦参碱可能通过活化p38MAPK来上调Fas、FasL的表达,从而促进Raji细胞凋亡。
Objective：To invest the effect and mechanism of matrine on apoptosis of human Burkitt′s lymphoma Raji cells.Methods：Raji cells were cultured in vitro and treated by different final concentrations（0.4,0.8,1.6 mg/mL） of matrine or combined with SB203580（p38 MAPK inhibitor） before matrine was added,then cocultured for 48 h,cell apoptosis rate was detected by AnnexinⅤ-FITC/PI double staining method and the P-p38 MAPK,Fas,FasL protein expresssion of Raji cells were evaluated by Western blot.Results：After cells were treated by matrine（0.4,0.8,1.6 mg/mL）,the corresponding total apoptosis rate（15.77±0.53）%,（27.88±1.52）%,（48.08±2.87）%,had statistical significance compared with SB203580 groups（11.48±0.64）%,（19.34±0.91）%,（33.98±1.26）%（P0.05 or P0.01）,and control group（8.78±0.66）%（P0.05 or P0.01）.As the concentration of matrine gradually increased,the protein expresssion levels of P-p38MAPK,Fas,FasL increased,and decreased after SB203580 were added,the correlation of P-p38MAPK and Fas,FasL was obvious.Conclusion：Matrine can upregulation of Fas and FasL to promote the apoptosis of Raji cells,it may be related to p38MAPK Activation.
出处
《中药材》
CAS
CSCD
北大核心
2011年第4期576-579,共4页
Journal of Chinese Medicinal Materials
基金
重庆市卫生局科研资助项目(2009-2-59)
