摘要
目的 通过活体动物实验,探究低频振动对骨髓基质干细胞（BMSC）骨向分化调控能力及其OPG、RANKL基因表达的影响.方法 BMSC结合骨基质明胶（BMG）移植联合低频振动刺激治疗骨缺损,随机分为非振动对照组和不同频率振动组,振动组于第7天开始接受振动干预5周,振动结束后对不同频率振动组别BMSC OPG、RANKL mRNA进行检测.结果 振动组BMSC OPG、RANKL基因表达明显上调（P＜0.05）,以25 Hz显著（P＜0.01）;100 Hz振动组BMSC OPG、RANKL基因表达下调（P＜0.05）.结论 低频振动可调控BMSC的骨向分化,可能通过调节OPG/RANKLmRNA的表达直接促进骨形成,抑制骨吸收.
Objective To explore the effect of low frequency vibration （LFV）on the osteogenic differentiation regulating capability of bone marrow stromal cell （BMSC） and the expressions of OPG （osteoprotegerin） mRNA and RANKL （nuclear factor kappa B ligand） mRNA through living animal experiment. Methods Both BMSC transplantation and low-frequency vibration were employed to treat bone defects. The groups were randomized into non-vibration and vibration of different frequencies. The vibration group received vibrating interventions at Day 7 for 5 weeks. After vibrations, the BMSC OPG and RANKL mRNA of different frequency groups were detected. Results The BMSC OPG and RANKL gene expressions significantly increased （ P ＜ 0. 05 ）, especially at 25 Hz （P ＜ 0. 01 ）. And for the vibration group at 100 Hz,the BMSC OPG and RANKL gene expressions decreased （ P ＜ 0. 05）. Conclusion Low-frequency vibration may promote the osteogenic differentiation capability of BMSC probably through regulating the OPG/RANKL mRNA expression, directly promoting bone formation and inhibiting bone resorption.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2011年第13期920-924,共5页
National Medical Journal of China
基金
国家自然科学基金（30970708）
关键词
骨髓移植
细胞外质
振动
基因表达
Bone marrow transplantation
Extracellular
Vibration
Gene expression
