摘要
背景：有研究表明姜黄素具有抗器官纤维化的作用，具抑制增殖及诱导G0／G1期细胞累积的作用，但姜黄索可否影响瘢痕疙瘩成纤维细胞的细胞周期，活化核转录因子kB通路，从而抑制瘢痕增宅至今少有报道。目的：观察姜黄素对体外培养人瘢痕疙瘩成纤维细胞周期的影响及其核转录因子kB信号转导通路的变化。方法：瘢痕疙瘩成纤维细胞进行体外原代培养，待细胞融合后传代，取第6-8代对数生长期的成纤维细胞用于实验将不匣浓度姜黄索（10，20，30，40，50，60IJmol／L）分别对瘢痕疙瘩成纤维细胞干预。用流式细胞仪测定细胞周期：免疫组织化学法检测核转录因子kB的表达。结果与结论：姜黄素呈剂量和时间依赖性抑制成纤维细胞的增生（P〈0.05），使细胞周期停滞在G1期，核转录因kB表逆随姜黄素剂量的增大而减小（P〈0.05）。结果显示，姜黄索能抑制瘢痕疙瘩成纤维细胞的增殖，并使细胞周期停滞往G1期，姜黄索可能通过抑制核转录因子kB信号转导通路的活化，从而发挥其机纤维化的作用。
BACKGROUND： Studies have indicated that curcumin can inhibit keloid fibroblasts. It can suppress keloid fibroblasts （KFB） proliferation and induce cell cycle arrest at G0/G1 phase. However, whether curcumin can inhibit KFB proliferation remains poorly understood. OBJECTIVE： To study the effect of curcumin on cultured human KFB proliferation and its signal transduction pathway. METHODS： Keloid fibroblasts were primary cultured in vitro, passaged after cell fusion, and 6 8 passages of cells with logarithmic growth were used. Different concentrations of curcumin （10, 20, 30, 40, 50, 60 pmol/L） were used to intervene keloid fibroblasts. Cell cycle was determined by flow cytometry, and expression of nuclear factor-KB was detected by immunohistochemistry. RESULTS AND CONCLUSION： Curcumin inhibited KFB hyperplasia in a dose-and time-dependent manner （P〈0.05）, and arrested cell cycle at G1 phase, nuclear factor-KB expression increased with the dose of curcumin decreased （P〈0.05）. Curcumin can inhibit KFB proliferation and arrest cell cycle in G~ phase. Curcumin might inhibit nuclear factor-KB signal transduction pathway activation, and thus play its anti-fiber role.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第11期2026-2029,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
