摘要
利用荧光探针和荧光素，研究了乌贼墨对Ｈ_(２２)癌细胞内 Ｃａ^（２＋）、ＡＴＰ浓度及线粒体Ｃａ^（２＋）／Ｍｇ^（２＋）－ＡＴＰ酶活性的影响。结果发现，乌贼墨使Ｈ_(２２)癌细胞内Ｃａ^（２＋）浓度降低了２．３和３．７倍，线粒体Ｃａ^（２＋）／Ｍｇ^（２＋）－ＡＴＰ酶活性降低了 ２８％和 ５８％，ＡＴＰ浓度升高 Ｔ ７７％和 ８３％。结果提示乌浓墨可能通过降低细胞内Ｃａ^（２＋）浓度，继而影响到线粒体上Ｃａ^（２＋）依赖性Ｃａ^（２＋）／Ｍｇ^（２＋）-ＡＴＰ酶活性，减少了Ｃａ^（２＋）向线粒体内的转运，减轻甚至消除了Ｃａ^（２＋）对ＡＴＰ合成的抑制作用。这可能是乌贼墨抑制肿瘤生长的机制之一。
The effects of Sepia on the intracellular Ca^(2+)、ATP concentrations and mitochondrial Ca^(2+)/Mg^(2+) -ATPase activities were studied in H_(22) cancer cells by using fluorescent probe and luciferin. The results showed that [ Ca^(2+) ] i decreased 2. 3 and 3. 7 times, mitochondrial Ca^(2+) /Mg^(2+) -ATPase activi ties diminished 28% and 58%, [ATP] i rose 77% and 83 %, respectively. The results suggested that Sepia probably diminished [ Ca^(2+) ] i, affected mitochondrial Ca^(2+) -dependent Ca^(2+)/Mg^(2+) -ATPase activi ties,thus reduced the amount of Ca^(2+) transported into mitochondria, lessened even eliminated the in hibitory effect of Ca^(2+) on ATP synthesis. This perhaps was one of the possible anti-cancer mechanisms of Sepia.
出处
《中国海洋药物》
CAS
CSCD
1999年第3期11-14,共4页
Chinese Journal of Marine Drugs
基金
卫生部科研基金!(94-2-135)
