摘要
目的： 探讨非小细胞肺癌神经内分泌分化的免疫组化诊断。方法： 选择三种神经内分泌共同标记ＣＧＡ、ＳＹＮ、ＮＣＮＭ， 应用免疫组化ＬＳＡＢ 法， 观察它们在ＮＳＣＬＣ 中的表达。结果： 在１６３ 例ＮＳＣＬＣ 中， １８ 例（１１－０４ ％ ） 表达ＣＧＡ，６３ 例（３８－６５ ％ ） 表达ＳＹＮ，４０ 例（２４－５４ ％ ） 表达ＮＣＡＭ 。７７ 例（４７－２４ ％ ） ＮＳＣＬＣ 至少有１ 种ＮＥ 标记表达，其中４０ 例只表达１ 种标记，３０ 例表达２ 种标记，仅７ 例３ 种标记均表达。３ 种标记表达彼此相关（ Ｐ＜０－００５） ，但存在差异（ Ｐ＜ ０－００５） 。与腺癌相比，腺鳞癌、鳞癌、大细胞癌染色阳性率较低。结论：选择３ 种ＮＥ 标记可以检测出ＮＳＣＬＣ 的ＮＥ 分化， 但不能以某一种标记的表达与否作为ＮＳＣＬＣ 的ＮＥ 分化诊断， 应联合检测以至少２ 种标记表达定义为ＮＥ 分化，在不同类型的ＮＳＣＬＣ 中腺癌更易出现ＮＥ 分化。
Objective:To study the immunohistochemical diagnosis of neuroendocrine differentiation in surgically resected NSCLC.Methods:Tumors from 163 patients with stage Ⅰ,Ⅱ, and ⅢNSCLC surgically treated in Tumor Hospital,Sun Yet sen University of Medical Seiences from January,1989 to August,1991 were analyzed immunohistochemically with antibodies to chromogranin A (CGA),synaptophysin(SYN),and neural cell adhesion molecule (NCAM).Results:The following percentages of cases were positive for each marker:CGA,11 04%;SYN,38 65%;NCAM,24 54%.Seventy seven(47 24%) were positive for ≥1 NE markers,of which only one marker was expressed in 40 cases, two markers in 30 cases,and three markers in only 7 cases. The expressions were significantly related among any two of the three markers (P< 0 005),but there existed differences (P< 0 005).When compared to adenocarcinoma,adenosquamous carcinoma,squamous cell carcinoma and large cell carcinoma displayed lower positive. Conclusion:The use of these three antibodies to CGA,SYN,NCAM may be recommended to detect the neuroendocrine differentiation of NSCLC,with the suggestion that reactivity with two or more antibodies should be required before neuroendocrine differentiation is accepted.Adenocarcinoma is more likely than any other histologic tumor types to express neuroendocine differentiation.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1999年第5期578-582,共5页
Chinese Journal of Cancer
关键词
非小细胞肺癌
神经内分泌
分化
病理诊断
Non small cell lung cancer
Neuroendocrine
Differentiation
Immunohistochemistry
Neural cell adhesion molecule
Chromogranin A
Synaptophysin
