摘要
目的：观察心肌细胞织反常时细胞内的游离钙浓度的变化，探讨丹参素和SOD对细胞内的游离钙浓度的影响。方法：体外分离成年大鼠心肌细胞复制氧反常模型，缺氧40min后给氧20min，用钙荧光探针（Fluo－3）分别测定了正常大鼠心肌细胞（Control，n=11）、缺氧40min（H40min，n=11）以及缺氧40min再给氧20min（H40minR20min，n=12）的大鼠心肌细胞内的游局钙浓度，并观察了丹参素（H40minR20min＋DS，n＝10)和SOD（H40minR20min＋SOD，n=11）对氧反常的心肌细胞内的游离钙浓度的影响，并用显微镜观察了细胞杆形率的改变。结果：氧反常组的细胞内的游离钙浓度（596．71±20．29nmol/L）明显比正常对照和单纯缺氧40min的高（158．67±5．01和328，51±13．86nmol/LP＜0.01）。加入丹参素和SOD，可明显降低氧反常细胞内的游离钙的浓度（分别为245.29±8．83和300．04±13.04nmol/L，P＜0．01）。细胞杆形率亦有类似的改变。结论：氧反常时心肌细胞发生明显的钙超载，丹参素和SOD对细胞内钙超载具有一定的防治作用。
To observe the changs of intracellular free calcium concentration in oxygen-paradoxed rat myocardial cells and to discuss the proective efforts of Salvia Miltiorrhizae (DS) and SOD on Ca2+ - overloaded rat myocardialcells. METHODS: Adult rat myocardial cells was isolated in vitro and oxygen paradox model was made. Selecting 40 minas hypoxia period followed by 20 min of reoxygenation, a new calcium fluoresence probe (Fluo - 3) was used to detect theintracellular free calcium concentration in control (n = 11) 、 H40min(hypoxia, n = 11 ) and H40minR20min (hypoxia+reperfuxion, n = 12) groups respectively. We also looked into the rod-shaped rates of myocardial cells with microscope.Inaddition, the effect of Salvia Miltiorrhizae (n = 10) and SOD (n = 11 ) on intracellular free calcium concentration and rod-shaped rate of oxygen-paradoxed myocedal cells were observed. RESULTS: The intracellular free calcium concentrationof H40minR20min group increased far beyond the control and H40min groups(596. 71 ± 20. 29 nmol/L vs 158. 67 ± 5. 01and 328.51 ± 13 .86 nmol/L, P < 0.01 ), showing a significant Ca2+ - overload. The changes of rod-shaped rates weresimilar to intracellular free calcium concentration. Salvia Miltiorrhizae and SOD can inhibit Ca2+ - overload obviously (254.29 ± 8. 83 and 300.04± 13 .04 nmol/L vs H40minR20min 596. 71 ± 20. 29 nmol/L, P < 0.01). Moreover, effect of Salvi-a Miltiorrhizae was more striking than SOD (P < 0.01 ). CONCLUSION: There is a significant Ca2+-overload in oxygen- peoxed myocedal cells. Salvia miltiorrhizae and SOD have a definite protective effect on Ca2+ - overload.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1999年第10期877-879,共3页
Chinese Journal of Pathophysiology
关键词
心肌缺血
氧反常
钙超载
丹参素
SOD
Myocardium
Oxygen
Calcium
Salvia Miltiorrhizae
Superoxide dismutase
