摘要
目的：用低硒／维生素E(Se／VE)饲料喂养的大鼠动物模型，施以冰冰在刺激，观察Se，VE对心肌血管活性物质的影响。方法：用60只Wistar大鼠，按体重和性别随机分为低硒／维生素EI组（Se/VEDGI）及上述每kg饲料补硒（亚硒酸钠）0.1mg（Se／VEDGI＋Se）组、补a－VE100mg（Se／VEDGI＋VE）组、联合补Se，VE（Se／VEDGI＋Se／VE）组、低硒／维生素EII组（Se／VEDGII）和常规饲料组（SG)等六组。饲养70d，测定各组动物心肌内皮素（ET），一氧化氮（NO），去甲管上腺素（NE），血管紧张素II（Ang－II），单胶氧化酶（MAO－A），超氧化物歧化酶（SOD），谷胱甘肽过氧化物酶（GSH－Px），脂褐素（lipofuscin）等水平。结果：Se／VEDG组心肌ET，Ang－II含量明显高于其它组；NO含量明显低于其它组；心肌NE水平增高，而特异性分解NE的MAO－A活性降低；心肌Lipofusein含量明显增加；SOD，GSH－Px含量降低。补Se组、补SE／VE组、低Se/VEII组和对照组上述指标活性明显改善。结论：冷刺激条件下硒和维生素E对大鼠心脏内血管活性物质有明显影响。
To study the effects of selenitum(Se) and vitmin E(VE) on vasoative subsboces in myocanhum ofrats under cold stress.METHOD: 60 Wistar rats were divided intO 6 groups: (1) Se, VE dificient I group (Se/VE DGI ); (2) Se supplemented 0.1 mg/kg group(Se/VE DG I + Se ); (3) VE supplemented 100 mg/kg group(Se/VE DG I+ VE ); (4) Se,VE supplemented group (Se/VE DG I + Se/VE ); (5) Se,VE dificient grain II group(Se/VE DG II );(6) Stock group(SG). Rats were fed for 70 days and were put in ice water stressed before killed. The contents of endothelin(ET), nitric oxide(NO), noradrinaline(NE), angiotensin-II(Ang-II), activihes of menoamine oxidase-A(MAO-A),superoxide dismutase(SOD), glutathione peroxidase(GSH-Px), and content of lipofuscin in myocardial were measured. RE-SULTS: Contents of ET, Ang- II and NE were abviously increased and contents of NO and activities of MAO-A, SOD,GSH-Px were markedly decreased in the myocedum of Se/VE DG I rats, but above items were improved in differentlygrade in those rats fed on Se/VE DG I supplemented Se,VE and Se/VE group. CONCLUSION: Se and /or VE playedimpotant role in some vasoactive substances of myocardium in rats fed on Se/VE dificient grain under cold stress.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1999年第10期871-873,共3页
Chinese Journal of Pathophysiology
基金
国家"八五"攻关课题!85-07-01-01
