摘要
目的 检测妇科肿瘤ｐ５３ 基因第５ ～８ 外显子的突变并探讨其临床意义。方法 用聚合酶链反应—单链构象多态性分析（ＰＣＲ－ ＳＳＣＰ）银染技术，检测了１４８ 例子宫平滑肌瘤、１６ 例子宫内膜癌、１５ 例宫颈癌、１７ 例卵巢癌的新鲜肿瘤组织ｐ５３ 基因第５ ～８ 外显子的突变。结果 子宫平滑肌瘤ｐ５３ 基因突变率为４．０５ ％ （６／１４８） ，第５～８ 外显子的突变率分别为０ 、１．３５ ％ 、０．６８ ％ 和２．０３ ％ 。突变组的发病年龄、临床症状、术中所见、术式选择及病理特征均与非突变组相似。子宫内膜癌ｐ５３ 基因突变率为１２．５ ％ （２／１６） ，宫颈癌为１３．３％ （２／１５） ，卵巢癌为２９．４ ％ （５／１７） 。结论 人子宫平滑肌瘤存在ｐ５３ 基因突变。
Objective To detect the mutation of p53 gene exons 5 to 8 in gynecologic tumors and to discuss its clinical significance.Methods Mutation of exons 5 to 8 of p53 gene in 148 patients with uterine leiomyoma,16 with endometrial carcinoma,15 with cervical carcinoma and 17 with ovarian cancer was screened,by the method of polymerase chain reaction -single strand polymorphism analysis (PCR-SSCP) with silver staining.Results Mutation of p53 gene was found in 6 cases(4 05%) of uterine leiomyoma.The rates of mutation of exons 5 to 8 were 0,1 35%,0 68% and 2 03% respectively .The patients' age,clinical syndrome,appearance of uterus,method of operation and pathologic features in mutation cases were almost the same as those of non-mutagenic uterine leiomyoma.Mutation of p53 gene was found in 2 cases(12 5%) of endometrial carcinoma,2 cases(13 3%) of cervical carcinoma,and 5 cases in ovarian cancer(29 4%).Conclusion p53 gene mutation exists in human uterine leiomyoma.Compared with the mutation rate of gynecologic malignant tumor,the rate of uterine leiomyoma is quite low.The clinical significance of p53 gene in leiomyoma may be less important.
出处
《中国实用妇科与产科杂志》
CAS
CSCD
北大核心
1999年第10期602-604,共3页
Chinese Journal of Practical Gynecology and Obstetrics
