摘要
邻苯二酚1,2-双加氧酶(EC 1.13.11.1)降解邻苯二酚为顺,顺-己二烯二酸,俗名粘康酸。它不仅用于合成热缩性树脂、感光性树脂及一些新功能树脂,而且是昂贵的电子材料和药用材料,由于它容易转化为己二酸,所以它也是生产尼龙的原料。用化学法制造顺,顺-己二烯二酸十分困难,生物法却较简单。目前,日本以苯甲酸为原料已大规模生产。本文报道臭味假单胞菌(Pse-
Catechol-1,2-dioxygenase(EC 1.13.11.1)catalyzes the degradation of catachol to cis,cis-muconic acid.The biochemical propertiesof catechol-1,2-dioxygenase from Pseudomon-as putida 84103 were investigated.The optim-um pH and temperature is 7.5—8.0 and 25—30℃,respectively.Cu^(2+),Zn^(2+) inhibit theenzyme activity.The paper chromatagraphand UV absorption spectrum of enzymaticreaction product are accordant with those ofthe standard muconic acid.
出处
《微生物学报》
CAS
CSCD
北大核心
1990年第5期397-399,共3页
Acta Microbiologica Sinica
关键词
臭味假单孢菌
加氧酶
Catechol-1,2-dioxygenase
cis,cis-muconic acid
Pseudomonas putida
