期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

在小麦上实施基因沉默的VIGS系统优化 被引量：4

Optimisation of Barley Stripe Mosaic Virus-induced Gene Silencing in Wheat
在线阅读 下载PDF
导出
摘要 以大麦条纹花叶病毒(Barley stripe mosaic virus,BSMV)改造的VIGS(Virus induced gene silencing)载体在单子叶植物中能够介导基因沉默,因此BSMV在植物基因功能鉴定中具有广泛的应用。本试验通过在近等基因系TcLr24中对BSMV VIGS载体系统进行优化,以保证更方便地在小麦近等基因系(NIL)中实施VIGS功能验证。 Virus induced gene silencing（VIGS） offers a rapid and high-throughput technical platform for gene function analysis using Barley stripe mosaic virus（BSMV）-based vectors in wheat.In this paper,we report optimization of BSMV-induced gene silencing in NIL TcLr24 in order to carry out VIGS in wheat.
作者 张立荣 杨文香 刘大群
机构地区 河北农业大学植物保护学院植物病理系
出处 《华北农学报》 CSCD 北大核心 2011年第2期111-113,共3页 Acta Agriculturae Boreali-Sinica
基金 国家自然科学基金项目(30771391)
关键词 大麦条纹花叶病毒 VIGS 基因功能鉴定 BSMV Virus-induced gene silencing Gene function characterization
分类号 Q78 [生物学—分子生物学]
  • 相关文献

参考文献12

  • 1陶小荣,周雪平,崔晓峰,钱亚娟.病毒诱导的基因沉默及其在植物基因功能研究中的应用[J].生物化学与生物物理进展,2004,31(9):777-783. 被引量：21
  • 2Burch-Smith T M, Anderson J C, Martin G B, et al. Applications and advantages of virus-induced gene silencing for gene function studies in plants [ J ]. The Plant Journal, 2004,39:734 - 746.
  • 3Boulcombe D C. Fast forward genetics based on virus-induced gene silencing[ J ]. Curr Opin plant Biol, 1999,2 : 109 - 113.
  • 4Holzberg S, Brosio P, Gross C, et al. Barley stripe mosaic virus-induced gene silencing in a monocot plant[ J]. Plant J ,2002:30,315 - 327.
  • 5Lacomme C, Hrubikova K, Hein I. Enhancement of virus-induced gene silencing through viral-based production of inverted-repeats [ J]. Plant J,2003,34:543 - 553.
  • 6Hein I, Barciszewska-Pacak M, Hrubikova K, et al. Virus-induced gene silencing-based functional characteriza- tion of genes associated with powdery mildew resistance in barley [ J ]. Plant Physilology, 2005,138:2155 - 2164.
  • 7Tai Y S, Jennifer Bragg, Michael C. Virus vector for gene silencing in wheat [ J ]. Bio Techiniques, 2005,39: 310 -314.
  • 8Scofield S R, Richard S N. Resources for Virus-Induced Gene Silencing in the Grasses [ J ]. Plant Physiology, 2009,149 : 152 - 157.
  • 9王宏芝,李瑞芬,王国英,马荣才,魏建华.病毒诱导的基因沉默及其在植物功能基因组学研究中的应用[J].自然科学进展,2005,15(1):8-14. 被引量：17
  • 10徐幼平,徐秋芳,宋晓毅,张志新,蔡新忠.病毒诱导的基因沉默[J].浙江大学学报（农业与生命科学版）,2008,34(2):119-131. 被引量：18

二级参考文献193

  • 1陶小荣,周雪平,崔晓峰,钱亚娟.病毒诱导的基因沉默及其在植物基因功能研究中的应用[J].生物化学与生物物理进展,2004,31(9):777-783. 被引量：21
  • 2刘晓东,张增艳,姚乌兰,辛志勇.在小麦上实施大麦条斑病毒诱导的基因沉默[J].作物学报,2005,31(11):1518-1520. 被引量：5
  • 3Dawson W O. Gene silencing and virus resistance: A common mechanism. Trends Plant Sci, 1996, 1:107.
  • 4Ratcliff F, et al. Gene silencing without DNA: RNA-mediated cross protection between viruses. Plant Cell, 1999, 11:1207.
  • 5Voinnet O. RNA silencing as a plant immune system against viruses. Trends Genet, 2001, 17:449.
  • 6Lu R, et al. Virus-induced gene silencing in plants. Methods,2003, 30:296.
  • 7Al-Kaff N S, et al. Transcriptional and post-transcriptional plant gene silencing in response to a pathogen. Science, 1998, 279:2113.
  • 8Ratcliff F, et al. A similarity between viral defense and gene silencing in plants. Science, 1997, 276:1558.
  • 9Chuang C H, et al. Specific and heritable genetic interference by double-stranded RNA in Arabidopsis thaliana. Proc Natl Acad Sci USA, 2000, 97: 4985.
  • 10Voinnet O, et al. Systemic signalling in gene silencing. Nature,1997, 389: 553.

共引文献59

同被引文献66

  • 1张怡,徐克东,杨松,李豪,曹鹏,魏森,韦丹丹,李成伟.蔊菜病毒诱导基因沉默体系构建[J].华北农学报,2013,28(6):65-70. 被引量：6
  • 2杨迎伍,李正国,宋红丽,杨平.VIGS技术在植物基因功能研究中的应用[J].植物生理学通讯,2007,43(2):379-383. 被引量：17
  • 3Burch-Smith T M, Anderson J C,Martin G B, et al. Applica-tions and advantages of virus-induced gene silencing for genefunction studies in plants[J]. The Plant Journal,2004,39(5):734-746.
  • 4BoucheN,Bouchez D. Arabidopsis gene knockout: phenotypeswanted[J]. Current Opinion in Plant Biology,2001 .4(2) :111-117.
  • 5FuD,Uauy C,Blechl A,Dubcovsky J,et al, RNA interferencefor wheat functional gene analysis[J]. Transgenic Research,2007,16(6):689-701.
  • 6ScofieldS R,Nelson R S. Resources for virus-induced gene si-lencing in the grasses [J]. Plant Physiology,2009,149 ( 1):152-157.
  • 7Purkayastha A. Dasgupta I. Virus-induced gene silencing: Aversatile tool for discovery of gene functions in plants [J].Plant Physiology and Biochemistry,2009,47 ( 11/12 ) : 967-976.
  • 8KumagaiM H, Donson J,Della-Cioppa G,et al. Cytoplasmicinhibition of carotenoid biosynthesis with virus-derived RNA[J]. Proceedings of the National Academy of Sciences of theUnited States of America,1995 ,92(5) : 1679-1683.
  • 9SahuP P,Puranik S.Khan M etal. Recent advances in tomatofunctional genomics: utilization of VIGS [J]. Protoplasma,2012,249(4):1017-1027.
  • 10BennypaulH S,Mutti J S,Rustgi S^et al. Virus-induced genesilencing (VIGS) of genes expressed in root,leaf. and meiotictissues of wheat [J]. Functional &- Integrative Genomics,2012,12(1):143-156.

引证文献4

二级引证文献18

华北农学报
2011年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部