摘要
油菜极易遭受各种病虫害的侵害,其中白斑病和黑斑病都属于真菌引起的病害。以优良品种花油6号为材料,以抗菌肽和B t融合基因为目标基因,以草甘膦为筛选标记基因,采用农杆菌介导的方法转入油菜获得转基因植株。经PCR和Southern检测表明外源基因已整合到油菜的基因组DNA中;经RT-PCR检测表明目的基因已在油菜转基因植株的转录水平表达;目的基因(B t-AMP)的遗传转化率为2.33%。研究结果可为油菜的抗性遗传改良提供新种质。
Rape is one of important oil crop that were widely grown in China.The diseases occures to rape easily,especially for white spot and black spot that caused by fungi.The variety of Huayou No.6 was used for tranferring Bt and Antibiotic peptide syncretic gene（Bt-AMP）,glyphosate as selective marker,by the method of agrobacterium-mediated transformation.The test of PCR and southern blot proved the exogenous DNA was integrated on the genome of rape;RT-PCR test showed the expression of transcriptional level of the introduced gene in rape plant.The tranformation rate of Bt-AMP was 2.33%.The research provided new germ for rape breeding of disease resistance.
出处
《华北农学报》
CSCD
北大核心
2011年第2期49-54,共6页
Acta Agriculturae Boreali-Sinica
基金
教育部"春晖计划"项目(Z2009-1-01065)
关键词
油菜
抗菌肽
BT
融合基因
转化
Rape
Antibiotic peptide
Bt
Syncretic gene
Transformation
