摘要
目的 探讨维拉帕米 (VP)对家兔肝缺血再灌注损伤的保护作用。方法 制备家兔肝缺血再灌注损伤模型 ,观察VP对血谷丙转氨酶 (GPT)、谷草转氨酶 (GOT)和血、肝组织黄嘌呤氧化酶 (XO)活性、丙二醛 (MDA)及肝细胞形态学变化的影响。结果 肝缺血再灌注期间 ,GPT、GOT、XO活性及MDA含量均明显升高 (P <0 0 5和P <0 0 1) ,肝细胞肿胀、空泡变性 ,线粒体、内质网明显肿胀 ;缺血前使用VP可明显减轻上述指标的异常变化 ,肝细胞形态学异常变化显著减轻。结论 缺血前使用VP具有良好的保护兔缺血再灌注损伤肝脏的作用。
Objective To explore the protective effect of verapamil (VP)in treating hepatic ischemia and reperfusion injury(HIRI).Methods Using a HIRI model in 30 rabbits,the different effects of VP on several parameters which included glutamate pyruvate transaminase(GPT)、glutamate oxaloacetate transminase(GOT) activites in the plasma and xanthine oxidase(XO) activities and malondialdehyde(MDA) contents in the plasma and liver tissue as well as hepatocellular changes morhologically were observed during HIRI.Results GPT、GOT and XO activities and MDA contents all increased very significantly( P <0 01),and there were obviously abnormal changes of liver cells morphologically during HIRI;VP adminisrered before ischemia,but not after ischemia,could obviously ameliorate the unnormal changes of parameters as above( P <0 05 and P <0 01),meanwhile unnormal changes of liver cells morphologically were remarkably alleviated.Conclusion VP administered before ischemia could effectively protect the liver frorm ischemia and reperfusion injury. [
出处
《中国急救医学》
CAS
CSCD
北大核心
1999年第10期592-594,共3页
Chinese Journal of Critical Care Medicine
基金
温州医学院科研基金
关键词
维拉帕米
再灌注损伤
肝缺血
家兔
Verapamil
Liver
Ischemia and reperfusion injury
Xanthine oxidase
