摘要
目的 调查强直性脊椎炎（ Ａ Ｓ） 病人中 Ｂ２７ 等位基因与其它 Ｈ Ｌ Ａ Ａ、 Ｂ 抗原之间的关系，为 Ａ Ｓ 与 Ｂ２７ 关联机理提供线索。方法 Ｈ Ｌ Ａ Ａ、 Ｂ 抗原分型采用 Ｎ Ｉ Ｈ 标准微量淋巴细胞毒方法， Ｂ２７ 等位基因的检测采用聚合酶链反应／ 顺序特异的寡核苷酸探针方法。对上海地区６８ 例 Ａ Ｓ 病人和３１８ 例正常对照进行调查。结果 Ａ Ｓ 病人根据其所检定的 Ｂ２７ 等位基因主要可分为 Ｂ２７０４和 Ｂ２７０５ 二大组， Ｈ Ｌ Ａ Ａ１１ 的频率在 Ｂ２７０４ Ａ Ｓ 组要比正常对照组显著增高（ Ｐ＝ ００００ ９８ ， Ｐｃ＜ ０ ．０１） ，在 Ｂ２７０５ Ａ Ｓ 组则比正常对照组稍低（ Ｐ＞ ０ ．０５） ， Ｂ２７０４ 与 Ｂ２７０５ Ａ Ｓ 组之间差异也有统计学意义（ Ｐ＜ ０ ．０５） 。结论 上海地区携带 Ｂ２７０４ 的 Ａ Ｓ病人与 Ｈ Ｌ Ａ Ａ１１ 抗原相关，提示 Ｂ２７０４ 与 Ｂ２７０５ 在 Ａ Ｓ 发病中的具体作用可能有所差异，或 Ｂ２７０４ 与 Ａ１１ 之间存在连锁不平衡。
Analysis of the relationship between HLAB27 alleles and HLAA, B antigens in ankylosing spondylitis patients$$$$ JIANG Lihua, YANG Jueqin, YAO Fangjuan, et al. Shanghai Second Medical University, Shanghai Institute of Immunology, Shanghai 200025 【 Abstract 】 Objective To investigate the relationship between HLAB27 alleles and other HLAA, B antigens in Shanghai Chinese patients with ankylosing spondylitis. Methods HLAA, B antigen typing was carried out in 68 B27 positive AS patients and 318 normal controls with the NIH standard microlymphocytoxicity method. HLAB27 alleles were assigned by using a PCR/SSO method. Results The dominant B27 alleles in AS patients were B*2704 and B*2705. The frequency of A11 was increased significantly in B*2704 AS patient group compared with the normal control group (Pc<0.01) and with the B*2705 AS group (P<0.05). Conclusions HLAA11 might be associated with B*2704 in AS patients from Shanghai area. Although this association may be explained by the linkage disequilibrium of HLAA11 with B*2704, the possibility that there might be some differences between B*2704 and B*2705 when they play a role on the pathogenesis of AS remains to be elucidated . Further investigation in larger samples especially in B27(+) healthy controls will be necessary 【 Subject words 】 Ankylosing spondylitis HLAA、B antigens HLAB27 gene
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1999年第5期403-405,共3页
Chinese Journal of Microbiology and Immunology
基金
国家自然科学基金
卫生部科学基金
