摘要
目的：探讨烧伤后早期腹腔巨噬细胞表达磷酸化酪氨酸（Ｔｙｒ Ｐ）、核因子 Ｋａｐｐａ Ｂ（ＮＦ κＢ）ｍ ＲＮＡ 及分泌细胞因子的动态变化。方法：采用小鼠２０％ 体表面积的Ⅲ度烫伤模型，分离和培养小鼠腹腔巨噬细胞。用免疫组织化学方法观察烫伤后２４ 小时内不同时间Ｔｙｒ Ｐ的变化；用逆转录聚合酶链反应（ＲＴＰＣＲ）方法观察ＮＦ κＢｍ ＲＮＡ表达的情况；用酶联免疫吸附（ＥＬＩＳＡ）方法测定上述细胞培养上清中肿瘤坏死因子 α（ＴＮＦ α）、白介素 ６（ＩＬ ６）的水平。结果：烫伤后１ 小时腹腔巨噬细胞的Ｔｙｒ Ｐ表达就明显增高，与假烫伤组比较差异显著（Ｐ＜ ０．０１），这种改变在伤后２４ 小时内持续存在；烫伤后１ 小时ＮＦ κＢｍ ＲＮＡ 表达明显增强，以伤后２ 小时为明显（Ｐ＜ ０．０１），伤后４、６ 小时表达逐步减弱，但仍较假烫伤组高（Ｐ均＜ ０．０５）；烫伤小鼠腹腔巨噬细胞培养上清中ＴＮＦ α和ＩＬ ６ 水平明显增高，烫伤后其含量在不同时期有所不同，与假烫伤组比较差异显著（Ｐ均＜ ０．０１）。烫伤小鼠腹腔巨噬细胞Ｔｙｒ Ｐ、ＮＦ κＢｍ ＲＮＡ 与ＴＮＦ α、ＩＬ ６ 呈正相关。结论：烫伤可使巨噬细胞中Ｔｙｒ Ｐ、ＮＦ κＢｍ ＲＮＡ 表达增强，分?
Objective:To observe the early dynamic changes in tyrosine phosphorylation (TyrP),nuclear factorκB (NFκB) mRNA and secretion of cytokines in peritoneal macrophages in severely burned mice.Methods:BALB/C mice were subjected to a 20 percent fullthickness scald injury.The peritoneal macrophages in different intervals postburn were obtained to measure TyrP and NFκB mRNA expression by immunohistochemical and reverse transcriptionpolymerase chain reaction analysis.The levels of tumor necrosis factorα(TNFα) and interleukin6(IL6) in the medium were determined by ELISA.Results:Significantly increased expression of TyrP at different intervals postburn was found,and NFκB mRNA expression markedly increased at 1 hour,peaking at 2 hours.The concentrations of TNFα and IL6 produced by macrophages were obviously higher in burned mice than in control mice.Positive correlations were found between TyrP,NFκB mRNA expression and TNFα,IL6 secretion.Conclusions:In the macrophages of the burned mice,the expression of TyrP and NFκB mRNA and the secretion of TNFα and IL6 were increased obviously.These changes may play an important role in mediating multiple organ damage by bacterial endotoxin.
出处
《中国危重病急救医学》
CSCD
1999年第10期591-593,共3页
Chinese Critical Care Medicine
基金
国家自然科学基金
关键词
烧伤
巨噬细胞
磷酸化酪氨酸
IL-6
细胞因子分泌
burns
macrophage
tyrosine phosphorylation
nuclear factorκB
tumor necrosis factorα
interleukin6
