摘要
以10 mmol/L CaCl2溶液处理滨梅幼苗叶片后,置于培养箱于(40±2)℃高温、光照强度(1 200±50)μmol·m-2·s-1下培养,定期测定有关生理生化指标,以探讨外源Ca2+对高温强光胁迫下滨梅幼苗的保护效应.结果显示:(1)与蒸馏水处理组相比,Ca2+处理使高温强光胁迫下滨梅幼苗叶片的脯氨酸含量显著升高,可溶性糖含量变化不明显,根系活力小幅降低;Ca2+处理有效抑制了高温强光下膜透性的加大,提高和保护了Ca2+-ATPase的活性.(2)采用Ca2+螯合剂EGTA或钙调素拮抗剂TFP对滨梅幼苗叶片同法处理并同条件胁迫时,与Ca2+处理相比,滨梅幼苗的脯氨酸、可溶性糖含量、Ca2+-ATPase活性和根系活力均明显下降,膜透性加大.研究表明,Ca2+处理能提高滨梅幼苗对高温强光的耐受性;Ca2+信号系统参与了胁迫过程中的渗透物质和Ca2+-ATPase活性等的调节.
After being sprayed with 10 mmol/L CaCl2,beach plum seedlings were cultured at（40±2）℃ in incubator,and the light intensity being（1 200±50） μmol·m^-2·s^-1,the correlative physiological indexes were measured regularly.The results show that：Ca^2＋ could evidently increace the content of proline and decrease root activity.It also shows that：Ca^2＋ could effectively inhibit cell membrane permeability increase the activity of Ca^2＋-ATPase.Moreover,addition of Ca^2＋ chelating agent EGTA or calmodulin antagonist TFP could markedly decrease the content of proline,soluble sugar,root activity,and the activity of Ca^2＋-ATPase.It also shows that：EGTA and TFP could obviously facilitate cell membrane permeability increase.Taken together the above results,it is suggested that Ca^2＋ could improve high temperature and strong light tolerance of beach plum seedlings and Ca^2＋ signal system may be regulated by change of some osmolytes and the activity of Ca^2＋-ATPase in beach plum seedlings under high temperature and strong light.
出处
《西北植物学报》
CAS
CSCD
北大核心
2011年第3期558-563,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
江苏省高校自然科学基础研究项目(08KJD210003)
关键词
CA2+
滨梅幼苗
高温强光
保护
Ca^2＋
beach plum seedlings
high temperature and strong light
protection
