摘要
用不同浓度Ca2+溶液浸种处理红三叶种子后、用0.1 mmol·L-1Cd2+溶液培养,探讨外源Ca2+对Cd2+胁迫下红三叶种子萌发、幼苗生长及其保护酶活性和子叶叶绿素含量的影响.结果显示:(1)0.1 mmol·L-1Ca2+浸种处理能显著缓解Cd2+胁迫的影响,可使红三叶种子发芽势、发芽指数及活力指数显著升高,全苗长、胚根长和胚根/胚芽显著增加(分别增加21.38%、44.06%和38.63%),并显著提高叶绿素含量(14.59%);(2)0.11、.0 mmol·L-1Ca2+浸种预处理均能显著提高Cd2+胁迫下红三叶幼苗子叶SOD活性(43.17%、218.95%)和POD活性(34.00%、14.28%),并显著降低其CAT活性(17.43%、29.19%)和MDA含量(21.92%、24.51%).结果表明,0.1mmol·L-1外源Ca2+浸种处理能显著缓解Cd2+(0.1 mmol·L-1)胁迫对红三叶种子萌发及幼苗生长及其保护酶活性的抑制作用,可明显提高红三叶幼苗对Cd2+胁迫的抵抗能力,缓解效果最优.
After seed-soaking with exogenous Ca^2＋ in different concentrations （0.1,1.0,10.0 mmol· L^-1 ） on seed germination, seedling growth, superoxide dismutase（SOD）, peroxidase （POD）, catalase （CAT） activities malondialdehyde（MDA） contents and chlorophyll contents of Trifoliurn pratense were studied under Cd^2＋ （0.1 mmol·L^-1 ） stress in order to investigate the buffer effect of Ca^2＋ on Cd^2＋ toxin. The results showed that. （1） 0.1 mmol·L^-2 Ca^2＋ could significantly reduce the damage of Ca^2＋ on Cd^2＋ toxin of T. pratense, notably increased germination energy, germination index and vigor index; seedling length, radicle length and ratio of radicles and plumules remarkably increased （21.38%, 44.065 and 38.63%, respectively ）, chlorophyll content obviously increased（14.59%）. （2） After seed-soaking with exogenous Ca^2＋ （0. 1,1.0 mmol·L^-1） ,the activities of SOD（43.17% and 218. 95% ,respectively） and POD（34.00%,14.28% ,respectively ） in leaves were notably increased, activities of CAT （17.43 %, 29.19 %, respectively） and MDA contents （21.92 %, 24.51% ,respectively） in leaves were significantly decreased; The results indicated that 0.1 mmol · L^-1 exogenous Ca^2＋ could decline the inhibition of Cd^2＋ stress （0. 1 mmol·L^-1 ）to seed germination and seedling growth of T. pratense ,could significantly enhance its resistant ability to Cd^2＋ stress,remission effect is the optimal.
出处
《西北植物学报》
CAS
CSCD
北大核心
2007年第9期1814-1819,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
教育部重点项目(105167)
陕西省重大科技专项(STXY-01-2)
西北农林科技大学校青年基金(08080266)
关键词
外源CA^2+
Cd^2+胁迫
红三叶
幼苗生长
生化特性
exogenous Ca^2＋
Cd^2＋ stress
Trifolium pratense
seedling growth
biochemical characteristics
