摘要
目的:研究依普利酮对大鼠单侧输尿管梗阻(UUO)模型肾间质纤维化的影响,探讨依普利酮的抗肾间质纤维化作用机制。方法:42只雄性Wistar大鼠随机分成假手术(SO)组、UUO组和UUO+依普利酮治疗组(T-UUO组,依普利酮100mg.kg-1.d-1)。于术后7d、14d分别处死各组7只大鼠。免疫组织化学法测定α-平滑肌肌动蛋白(α-smoothmuscle actin,α-SMA)、单核细胞趋化蛋白-1(monocyte chemoattractant protein-1,MCP-1)、单核细胞/巨噬细胞-1(monocytes/macrophages-1,ED-1),及增殖细胞核抗原(proliferative cell nuclear antigen,PCNA)的表达。结果:UUO组肾间质α-SMA、MCP-1、ED-1、PCNA的表达较SO组增加(P<0.05);在术后各时间点,使用依普利酮治疗的T-UUO组大鼠肾间质α-SMA、MCP-1、ED-1、PCNA的表达较UUO组显著减少(P<0.05),但仍高于SO组(P<0.05)。结论:依普利酮可通过降低α-SMA和MCP-1表达、抑制单核/巨噬细胞浸润和和系膜细胞增生,减轻肾间质纤维化。
Objective：To investigate the effect of eplerenone to fibrosis of renal interstitial in rats following unilateral ureteral obstruction,and explore the possible mechanism of anti-fibrosis of renal interstitial.Methods：42 male Wistar rats were distributed casually into sham-operated-groups（SO）and unilateral ureteral obstruction operated groups（UUO）and unilateral ureteral obstruction operation with eplerenone treatment groups（T-UUO,eplerenone 100mg·kg-1·d-1）.Seven rats of each group were killed respectively at 7,14 days after unilateral ureteral obstruction operation.Immunohistochemistry were performed to measure level of expression of a-smooth muscle actin（α-SMA）,monocyte chemoattractant protein-1（MCP-1）,monocytes/macrophages-1（ED-1）,proliferative cell nuclear antigen（PCNA）.Results：The level of expression of interstitial α-SMA、MCP-1、ED-1、PCNA showed a marked increase in UUO when compared to SO（P0.05）.At every time point after operation,in T-UUO were treated by eplerenone,the level of expression of interstitial α-SMA、MCP-1、ED-1、PCNA showed also a marked decrease when compared to UUO（P0.05）.But it still showed increase when compared to SO（P0.05）.Conclusion：Eplerenone inhibit renal interstitial fibrosis by decreasing the level of expression of α-SMA and MCP-1,and suppressess infiltration of monocyte/macrophage and mesangial cell proliferation.
出处
《中国中西医结合肾病杂志》
2011年第2期108-111,190,共5页
Chinese Journal of Integrated Traditional and Western Nephrology
基金
广东省医学科研基金立项项目(No.A2010468)
广州市医药卫生科技一般引导项目(No.2009-YB-044)
关键词
依普利酮
肾间质
纤维化
单侧输尿管梗阻
Eplerenone Renal interstitial Fibrosis Unilateral ureteral obstruction
