摘要
目的 探讨 Ｍ Ｄ Ｒ１ 基因表达与原代细胞体外抗药性的关系，为原发上皮性卵巢癌合理化疗提供理论依据。方法 应用半定量逆转录———聚合酶链反应技术和 Ｍ Ｔ Ｔ 法体外药敏试验，检测了３７ 例原发上皮性卵巢癌原代细胞对临床常用抗癌药物的抗药性及其 Ｍ Ｄ Ｒ１基因表达情况。结果 原发上皮性卵巢癌 Ｍ Ｄ Ｒ１ 基因阳性表达率为３０ ％ 。 Ｍ Ｄ Ｒ１ 基因表达与 Ｍ Ｄ Ｒ 相关药物耐药有关，与非 Ｍ Ｄ Ｒ 相关药物耐药无关。结论 Ｍ Ｄ Ｒ１ 基因表达是原发上皮性卵巢癌抗 Ｍ Ｄ Ｒ 相关药物的主要原因。
Objective To study the relationship between in vitro chemosensitivity of primary epithelial ovarian carcinoma (PEOC) to MDR related drugs and expression of MDR 1 gene.Methods A sensitive semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) assay was used for detection of MDR 1 gene expression in 37 PEOC .Sensitivity of the tumor tissues to the clinical common anticancer agents was examined by means of MTT assay in vitro.Results MDR 1 gene expression was detected in 30% of PEOC analysed.The level of MDR 1 mRNA was not correlated with nonMDR related drugs, but correlated with MDR related drugs.Conclusion These results support the idea that the increased expression of MDR 1 gene is a major cause of resistance to MDR related drugs.
出处
《济宁医学院学报》
1999年第3期10-12,共3页
Journal of Jining Medical University
关键词
卵巢肿瘤
基因表达
抗药性
比色法
Ovarian neoplasma
Drug resistance
multiple
Gene expression
Polymerase chain reaction
Colorimetry.
