摘要
目的研究柴胡龙牡汤对Lewis肺癌小鼠T细胞亚群、NK细胞活性及IFN-γ、IL-10水平的影响。方法将Lewis肺癌细胞株接种于C57BL/6J近交系小鼠右侧腋部皮下,造模成功后随机分为模型组(生理盐水)、中药组(柴胡龙牡汤)、化疗组(顺铂)、综合组(柴胡龙牡汤+顺铂)分别给药。剥离肿瘤,称重计算抑瘤率;采用乳酸脱氢酶(LDH)释放实验检测小鼠脾脏NK细胞活性;采用双抗体夹心ELISA法检测小鼠脾上清液中IFN-γ、IL-10的含量;运用流式免疫荧光技术检测小鼠脾T淋巴细胞亚群。结果综合组抑制肿瘤细胞生长的作用最为显著,抑瘤率高达62.4%,与化疗组或中药组比较,差异均有统计学意义(P<0.01)。中药组NK细胞活性最高,与模型组、化疗组及综合组比较,差异均有统计学意义(P<0.01)。中药组IFN-γ含量为65.78±17.68,高于化疗组的50.69±10.12,差异有统计学意义(P<0.05)。模型组IL-10含量在4组中最高,为153.30±33.14,中药组IL-10含量则明显低于模型组、化疗组及综合组,差异有统计学意义(P<0.05)。中药组小鼠CD4+及CD4+/CD8+比值最高,明显高于模型组和化疗组,差异有统计学意义(P<0.01);治疗后各组小鼠脾脏CD8+细胞的百分比无明显变化。结论柴胡龙牡汤具有抑制肿瘤生长的作用;此外,还可通过提高Th1型细胞因子IFN-γ的水平,降低Th2型细胞因子IL-10的优势表达,增强NK细胞活性,显著升高CD4+细胞比例及CD4+/CD8+比值,改善机体细胞免疫功能。
Objective To study effects of Chaihulongmu decoction on cellular immune function such as T lymphocyte subgroup,NK cell activeness,IL-10 and γ-interferon of Lewis mice with lung cancer. MethodsThe cells of Lewis lung cancer were planted in the right axilla of C57BL/6J inbred strain mice subcutaneously.The mice with cancer were randomly divided into model group（MG）,herbal group（HG）,DDP group（DG） and combination group（CG）.The tumors were weighed when the mice were sacrificed in each group.Then the inhibition rate of tumor was calculated.The experiment of the lactic dehydrogenase（LDH） releasing was used to detect the activity of NK cell in the mouse spleens.The method of enzyme-linked immuno sorbent assay（ELISA） was used to detect the IL-10,γ-interferon in the supernatant fluid of spleen.The rates of CD4,CD8 and the ratio of CD4/CD8 were detected by the flow cytometry（FCM） in the way of PE/FITC fluorescent staining in the spleens of mice. ResultsThe inhibited effectiveness of CG was optimal,and the inhibition rate of tumor was 62.4%.There was significant difference comparing to DG and HG（P0.01）.The activity of NK cell in HG was highest,and the difference was obvious comparing to MG,DG and CG（P0.01）.The content of IFN-γ was 65.78±17.68 in HG,which was higher than that in DG（P0.05）.The content of IL-10 in MG was highest（153.30±33.14）.In HG it was lower than MG,DG and CG（P0.05）.The figure of CD4＋ and the ratio of CD4＋/ CD8＋ in HG were the highest.Comparing to the MG and the DG,the difference was extremely obvious.（P0.01）.While the percentage of CD8＋ cell in each post-treatment group had no obvious changes. ConclusionThe prescription of Chaihulongmu decoction can inhibit tumor and improve the cellular immune function through raising the level of IFN-γ,reducing the superior expression of IL-10,strengthening the activity of the NK cell,obviously elevating the percentage of the CD4＋cell and the ratio of CD4＋/CD8＋.
出处
《临床肿瘤学杂志》
CAS
2011年第3期211-215,共5页
Chinese Clinical Oncology
