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抗癌药物L-天冬酰胺酶摇瓶发酵研究 被引量:4

Studies on Fermentation of Anti cancer Drug—L asparaginase in the Shake Flask
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摘要 对大肠杆菌 L- 天冬酰胺酶的摇瓶发酵进行了研究。在最适条件下发酵 12h,可使产酶达到60u/m l。在培养基中添加 001% 硫酸亚铁和 2% 的 W 试剂可分别提高产酶能力 15% 和28% 。该发酵过程是一个耗氧很大的过程。 The fermentation of E .coli L asparaginase in shake flask is discussed. FeSo 4 Under the optimal culture conditions,the L asparaginase activity achieved 60u/ml.When the addition of is 0 01% and the addition of W agent is 2% in the mediums,the L asparaginase activity is increased respectively by 15% and 28%.The fermentation procss of L asparaginase is a process of large oxygen requirement.
出处 《四川轻化工学院学报》 1999年第3期60-64,共5页 Journal of Sichuan Institute of Light Industry and Chemical Technology
基金 四川省教委重点科研项目
关键词 抗癌药物 天冬酰胺酶 摇瓶发酵 发酵 anti cancer drug L asparaginasr fermentation in shake flask
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