摘要
以抗癌药物L－天冬酰胺酶生产为应用背景，针对发酵过程中存在的严重耗氧问题，研究了氧载体对发酵过程的影响。通过对几种氧载体的筛选，认为正十二烷最适合该发酵过程。随后以产物L－天冬酰胺酶活性、菌体浓度以及溶氧水平为主要指标，考察了氧载体在发酵过程中的作用，实验表明，发酵基质中5％正十二烷的添加量为最佳浓度，这种氧载体的加入，明显地提高了发酵介质中的溶氧水平，改善了供氧条件，增加了菌体浓度，提高了L－天冬酰胺酶发酵水平，在优化条件下，可使发酵液最终酶活提高21％左右。
Production. of L-asparaginase as one of the anti-cancer pharmaceuticals is involved in the paper as application background. Effect of oxygen-vector on fermentation process is investigated in order to improve oxygen supply. N-dodecane is the most effective to the related process among tested oxygen-vectors. Activity of L-asparaginase,concentration of cells and level of dissolved oxygen are regarded as main indexes for estimating the effectiveness of the oxygen-vector to this fermentation process. It is demonstrated that level of dissolved oxygen and concentration of cells are increased markedly by addition of 5 %n-dodecane as oxygen-vector in the fermentation medium. Under optimal conditions, productivity of L-asparaginase is also improved more than that without oxygen-vector by 21 %.
出处
《四川轻化工学院学报》
1998年第2期25-29,共5页
Journal of Sichuan Institute of Light Industry and Chemical Technology
基金
四川省教委青年基金
