摘要
目的:近年研究认为精子DNA碎片化是评价精子质量的重要指标,本研究主要运用吖啶橙实验(acridine orangetest,AOT)检测不育症患者精子DNA碎片化指数(DNA fragmentation index,DFI)与精子常规参数之间的关系。方法:收集本院男科门诊376例不育症患者的精液标本,采用改良的Neubauer血细胞计数板人工分析精子密度,计算机辅助精液分析(CASA)分析精子活力。精子形态分析采用Shorr染色法,分析标准采用WHO推荐的Tygerberg严格标准。采用AOT检测精子DNA碎片化程度。结果:DFI与精子密度呈负相关(r=-0.150,P=0.003)、与精子总活动率呈负相关(r=-0.114,P=0.028)、与精子前向运动率无统计学相关性(r=-0.089,P=0.085)、与精子正常形态率呈负相关(r=-0.155,P=0.003)。结论:精子DNA碎片化指数与精子的密度、总活动率及精子正常形态率具有负相关性,AOT检测的DFI值为男性不育症的诊疗提供了一个新的指标。
Objective：Sperm DNA fragmentation has been assessed as a positive predictor of fertility potential in recent years.The aim of this study is using the acridine orange test（AOT） to analyze the relationship between sperm DNA fragmentation indexes（DFI） and semen conventional parameters of infertility men.Methods：Semen samples were collected from 376 infertility male patients in our andrology outpatient department.The samples were analyzed by improved Neubauer haemocytometer chamber and computer-aided sperm analysis（CASA） system to obtain concentration and motility parameters.All the samples were stained by Shorr staining method and the smears were analyzed according to Tygerberg strict criteria recommended by World Health Organization（WHO） to obtain morphology parameters.The DFI as marker of sperm DNA damage determined using the AOT.Results：Significant negative correlations were noted between DFI and sperm concentration（r=-0.150,P=0.003）,motility（r=-0.114,P=0.028）and morphology（r=-0.155,P=0.003）.No significant correlations were found between DFI and sperm progressive motility（r=-0.089,P=0.085）.Conclusion：Sperm assessment for DNA status using AO is reliable and shows good correlation with sperm count,motility and morphology.Assessment of sperm DNA fragmentation with AOT measures a new parameter of male fertility.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2011年第4期565-567,570,共4页
Journal of Nanjing Medical University(Natural Sciences)
基金
国家自然科学基金(81070485)
关键词
精子
染色质
精液分析
不育
男性
sperm
chromatin
semen analysis
infertility
male
