摘要
目的:建立人源化CD52单克隆抗体诱导食蟹猴的动物模型。方法:使用流式细胞仪筛选出红细胞表面不表达CD52抗原的食蟹猴,并观察给药后不同时间血中淋巴细胞数量变化情况。结果:食蟹猴血中淋巴细胞呈现出快速被清除及恢复的过程。CD20+B淋巴细胞、CD8+T淋巴细胞、CD4+T淋巴细胞分别在给药后213、5和56 d恢复至给药前水平。结论:食蟹猴外周血中淋巴细胞清除效果略低于临床病人,恢复速度较快。本实验模型可用于临床人源化CD52单克隆抗体器官移植前的实验研究。
Objective： To establish the model of alemtuzumab used for induction therapy in cynomolgus monkey.Methods： Monkeys without CD52 antigen on erythrocytes were selected and lymphocyte depletion and repopulation in peripheral blood was documented.Results： Powerful depletion of lymphocytes from blood followed by gradual repopulation was observed.CD20＋ B cells,CD8＋ T cells,CD4＋ T cells returned to pretreatment levels by day 21,35,56. Conclusion： Depletion of lymphocytes in peripheral blood was less powerful and repopulation occurred faster than in patients.This model can be used in preclinical transplantation.
出处
《肠外与肠内营养》
CAS
北大核心
2011年第2期102-105,共4页
Parenteral & Enteral Nutrition
基金
"十一五"国家科技支撑计划(2008BAI60B06)
江苏省创新学者攀登项目(BK2008034)
