摘要
目的：探讨声波对离体耳蜗内外毛细胞（ Ｏ Ｈ Ｃ）内游离 Ｃａ２＋ 浓度（〔 Ｃａ２＋ 〕ｉ）的影响。方法：采用荧光染色法，用 Ａ Ｃ Ａ Ｓ５７０ 型粘附式细胞仪对纯音刺激引起的豚鼠耳蜗 Ｏ Ｈ Ｃ的〔 Ｃａ２＋ 〕ｉ的变化进行动态观察。结果：声刺激可引起〔 Ｃａ２＋ 〕ｉ明显升高，至高峰后迅速下降，再次刺激可引起类似反应。结论：声音刺激可引起单个 Ｏ Ｈ Ｃ的生理变化。
Objective:To study the effects of acoustic stimuli upon intracellular free calcium concentration (〔Ca2+〕i) of cochlear outer hair cells (OHCs) isolated from guinea pigs.Method:The specimens were stained with fluorescence,and measured by using ACAS 570.Result:Acoustic stimuli caused a remarkable increase of 〔Ca2+〕i and the concentration of 〔Ca2+〕i decreased gradually after reaching its peak value.The reponeses can repeat with another stimuli.Conclusion:The acoustic stimuli could alter the physiologic characteristics of single OHC.
出处
《临床耳鼻咽喉科杂志》
CSCD
1999年第8期359-360,共2页
Journal of Clinical Otorhinolaryngology
关键词
耳蜗
外毛细胞
钙
声波
Cochlea Outer hair cells Calcium Acoustic wave
