摘要
采用直接进样毛细管气相色谱法测定鱼油脂肪乳注射液中DHA和EPA的含量,色谱柱为PEG-20M毛细管柱,柱温采用程序升温,载气为氦气,流速0.5 mL/min,采用FID检测器,温度为280℃,进样口温度300℃,分流比5∶1,进样量1μL。结果表明,DHA甲酯及EPA甲酯浓度分别为0.197～0.690 g/L 0,.208～0.743 g/L时线性关系良好r,分别为0.999 50,.998 4;平均回收率分别为99.99%9,9.41%,RSD分别为0.52%,0.47%。该法简便、准确,重现性好,可用于鱼油脂肪乳注射液中DHA和EPA的含量测定。
The content of DHA and EPA in fish oil lipid emulsion injection was determined by capillary gas chromatographic method with directly sampling.The chromatographic column was PEG-20M.The column temperature was programmed.The carrier gas was helium and the flow rate was 0.5 mL/min.The detector was FID and its temperature was 280 ℃.The injection temperature was 300 ℃,the split ratio was 5 ∶ 1,and the injection volume was 1 μL.Results showed that the linear rang of DHA ester and EPA ester was 0.197~0.690 g/L and 0.208~0.743 g/L,r was 0.999 5 and 0.998 4,respectively.The average recovery was 99.99% and 99.41%,RSD was 0.52% and 0.47%,respectively.The method was simple,accurate and reproducible and it can be used to determinate the concentration of DHA and EPA.
出处
《应用化工》
CAS
CSCD
2011年第3期542-544,共3页
Applied Chemical Industry
