摘要
目的建立一种稳定的大鼠原位肾移植慢性排斥反应模型。方法供体为近交系F344大鼠,受体为Lewis大鼠,供肾采用左肾,在体修整,原位灌注。肾静脉用硬膜外导管做为临时内支架管端-端、六针法吻合,腹主动脉端-侧连续缝合,输尿管带膀胱瓣与膀胱吻合。受体术前3 d开始环孢素A灌胃至术后30 d(5 mg/kg·d),以预防急性排斥反应。结果手术时间120～180 min;手术成功率90.9%(40P44);受体均存活60 d。并发症有吻合口出血、静脉血栓形成、急性排斥反应等。结论供肾原位灌注,在体修整是简单可靠的方法。静脉內支架管端端吻合,腹主动脉端侧吻合能够达到稳定的成功率,值得推广。熟练的外科操作技能和血管吻合技术是手术成功的关键。
Objective To explore establishment a stable model of rat chronic renal allograft rejection.Methods Inbred F344 and Lewis rats were used as the donors and the recipients.Used the left kidney as a donor kidney,dressing for the kidney in vivo and in situ perfusion.Epidural catheter within the renal vein as a temporary stent tube,end-to-end of the six acupuncture anastomosis.Artery end-to-side continuous suture;ureter and bladder with the bladder flap anastomosis,Used cyclosporine A 5 mg /kg.d to 30 days after operation to prevent acute rejection from three days before operation.Results The total time of operation about 120 ～ 180 min,success rate about 90.9%（40P44）;receptors can survive up to 60 d.the complications include anastomotic bleeding,venous thrombosis and acute rejection etc.Conclusion Dressing for kidney in vivo and in situ from renal perfusion is a simple and reliable method.Stent vein of end-to-end anastomosis,abdominal aortic end-to-side anastomosis can be achieved stable rate of success is worth promoting.Skilled surgical and vascular anastomosis operation is the key to success.
出处
《中国比较医学杂志》
CAS
2011年第3期18-21,F0002,79,共6页
Chinese Journal of Comparative Medicine
关键词
肾移植
大鼠
模型
动物
慢性排斥反应
Renal transplantation
Rats
Model
animal
Chronic rejection
