摘要
目的本文旨在研究NICD与COX-2在胃癌组织中的表达及其临床生物学意义。方法通过免疫组化方法检测109例胃癌组织和57例胃癌前病变组织及72例慢性浅表性胃炎组织中NICD和COX-2的表达,分析两者的表达水平与临床病理特征的关系以及两者的相互关系。结果 NICD在胃癌组中的阳性率为74.31%(81/109),明显高于癌前病变组31.58%(18/57)及慢性浅表性胃炎组23.61%(17/72)(χ2=53.45,P<0.01),与肿瘤大小、分化程度、浸润深度、淋巴结转移有关(χ2=5.51,P<0.05;χ2=4.76,P<0.05;χ2=4.44,P<0.05;χ2=4.62,P<0.05)。COX-2在胃癌组中的阳性率为71.56%(78/109),明显高于癌前病变组1.75%(1/57)及慢性浅表性胃炎组0.00%(0/72)(χ2=133.50,P<0.01),与肿瘤的分化程度、淋巴结转移有关(χ2=20.78,P<0.01;χ2=4.15,P<0.05)。NICD和COX-2在胃癌中表达呈正相关(r=0.30,χ2=9.38,P<0.01)。Ka-plan-Meier分析显示:NICD阳性组2年生存率50.50%,阴性组2年生存率72.80%;COX-2阳性组2年生存率55.10%,阴性组2年生存率71.40%;NICD阳性COX-2阳性组、NICD阳性COX-2阴性组、NICD阴性COX-2阳性组、NICD阴性COX-2阴性组2年生存率分别为43.10%、100.00%、66.7%、100.00%。生存曲线的log-rank检验显示:NICD与COX-2阳性对胃癌患者生存率影响有统计学意义(χ2=9.70,P<0.01;χ2=7.95,P<0.01)。Cox回归分析显示:NICD与COX-2可以作为影响胃癌预后的独立因素(χ2=7.55,P<0.05;χ2=4.45,P<0.05)。结论 NICD、COX-2在胃癌的发生发展过程中可能起着促癌作用,这两项指标的表达水平可作为评价胃癌预后的指标,联合检测这两项指标在评估胃癌预后方面具有一定的临床价值。
Objective To investigate the expression and significance of NICD and COX -2 in gastric cancer. Methods The expression of NICD and COX- 2 in a tissue microarray containing 109 cases of gastric cancer tissues,57 cases of precancerous lesions and 72 cases of chronic superficial gastritis was detected by SP immunohistochemistry. The correlation of NICD expression and COX - 2 expression,and their correlation to the clinicophathologic features of gastric cancer was analyzed. Results The postive rate of NICD was significantly higher in gastric cancer than that in precancerous lesions and chronic superficial gastritis （74. 31% vs 31. 58% ,74. 31% vs 23.61% separately ;X2 = 28.39,P 〈 0.01 ;X2 = 44.89,P 〈 0.01 ） , and closely related to tumor size （X2 = 5.51, P 〈 0.05 ） , differentiation grade （X2 = 4.76,P 〈 0.05 ） , depth of invasion （X2 = 4.44, P 〈 0.05 ） and lymph node metastasis （X2 = 4.62, P 〈 0.05 ）. The positive rate of COX - 2 was significantly higher in gastric cancer than in precancerous lesions and chronic superficial gastritis （71.56% vs 1.75% ,71.56% vs 0. 00% separately;A,2 = 73. 12, P 〈 0.01 ,X2 = 90.54, P 〈 0.01 ） , and closely related to differentiation grade （X2 =20.78,P 〈 0.01 ） and lymph node metastasis（X2 = 4.15,P 〈 0.05）. The expression of NICD was positively correlated to that of COX - 2 （ r = 0.30,X2 = 9.38 ,P 〈 0.01 ）. The 2 - year survival rate was significantly higher in NICD（ - ） group （ NICD - negative） than in NICD （ ＋ ） group （ NICD - positive） （72.80% vs 50.50% ;X2 = 9.70, P 〈 0.01 ）, and was significantly higher in COX - 2 （ - ） group （ COX - 2 - negative） than in COX - 2 （ ＋ ） group （ COX - 2 - positive） （71.40% vs 55.10% ;X2 = 7.95, P 〈 0.01 ）. The 2 - year survival rates of NICD（ ＋）COX-2（＋） group, NICD（＋） COX -2（-） group, NICD（-） COX -2（＋） group,and NICD（-） COX -2（-） group were 43.10% , 100.00% ,66.7% , 100.00% separately. COX regression analysis showed that NICD expression and COX - 2 expression were independent prognostic factors of gastric cancer（x2 = 7.55,P 〈 0.05 ;X2 = 4.45 ,P 〈 0.05 ）. Conclusion The dys - regulation of NICD and COX - 2 expression may correlate to the occurrence and development of gastric cancer. NICD and COX - 2 may be novel prognostic markers of gastric cancer. Combined detection of these two indicators has some clinical value in assessing the prognosis of gastric cancer.
出处
《医学研究杂志》
2011年第3期61-66,共6页
Journal of Medical Research
基金
浙江省自然科学基金资助项目(Y2101458)
