摘要
目的:通过探讨人脐带间充质干细胞(hUC-MSCs)低氧条件培养液对小鼠造血系统自然衰老的延缓作用,进一步了解hUC-MSCs对机体造血的支持作用机制。方法:将6月龄的BALB/c小鼠随机分为实验组和对照组,前者腹腔注射hUC-MSCs低氧条件培养液,每4 d 1次,持续6个月;后者注射培养基。干预后观察小鼠一般情况,并于干预后第3个月和第6个月分别比较两组的外周血象(白细胞与血红蛋白)、单侧股骨骨髓有核细胞(BMCN)计数、造血祖细胞集落(CFU-GM、CFU-E、CFU-MK)培养、成纤维细胞集落(CFU-F)培养和外源性脾集落形成单位(CFU-S)计数。于第6个月时观察骨髓病理变化、测定比较骨髓细胞重建造血能力及P16蛋白表达情况。结果:实验组小鼠的一般情况优于对照组;干预后第3个月,实验组的BMNC、CFU-GM和CFU-MK多于对照组,而外周血象、CFU-F和CFU-E无明显差别;干预后第6个月,外周血象仍无显著差别,其余的上述指标实验组均明显高于对照组,且各组的这些指标随时间变化呈不断下降的趋势,但实验组的下降速度明显慢于对照组,差异显著(P<0.05)。组织学观察发现,实验组造血组织较对照组丰富,P16蛋白的表达也明显低于对照组,而对照组骨髓脂肪化显著增加;实验组骨髓细胞的造血重建能力明显优于对照组。结论:hUC-MSCs分泌的细胞因子能够延缓小鼠造血系统的衰退,有望应用于延缓衰老的研究。
AIM： To explore the anti-aging roles of hypoxic-conditioned medium of mesenchymal stem cells from human umbilical cord（hUC-MSCs） on the natural senescence of hematopoietic system in aging mice. METHODS： The 6-month-old BALB/c mice were randomly divided into experimental group and control group.The mice in experimental group were intraperitoneally injected with hypoxic-conditioned medium of hUC-MSCs every 4 days for 6 months,and the mice in control group were injected with medium.After treated with hypoxic-conditioned medium of hUC-MSCs,the general situations of the mice were observed.Three months and 6 months after treatment,peripheral hemogram（white blood cell and hemoglobin）,bone marrow nucleated cell（BMNC）count,hematopoietic progenitor cell colony（CFU-GM,CFU-E,CFU-MK）culture,fibroblast colony（CFU-F）culture,and exogenous spleen colony-forming unit（CFU-S）count were compared between the 2 groups.Bone marrow histology,P16 expression,and the capability of hematopoietic reconstruction of the bone marrow cells were also compared at the sixth month. RESULTS： The general status of the mice in experimental group was better than that in control group.At the third month,BMNC,CFU-GM and CFU-MK of the experimental group were higher than those in control group.No significant difference of the peripheral hemogram,CFU-F,CFU-E and CFU-S was observed.At the sixth month,all the indexes mentioned above in experimental group were higher than those in control group except peripheral hemogram.All the indexes had a similar descending-tendency during 6 months,but the tendency of experimental group was much slower than that of control group with significant difference（P〈0.05）.In experimental group,more hematopoietic tissue and less adipose tissue were observed,and the expression level of P16 was lower than those in control group.The hematopoietic reconstruction capability of bone marrow cells in experimental group was better than that in control group. CONCLUSION： The cytokines from hUC-MSCs slow down the natural recession of hematopoietic system in aging mice,which may be considered to be a potential strategy of anti-aging research in the future.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2011年第3期469-474,共6页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.30670902)
关键词
间充质干细胞
脐带
衰老
细胞因子类
Mesenchymal stem cells
Umbilical cord
Aging
Cytokines
