摘要
目的:探讨染色体3p区域多个等位基因位点的杂合性缺失(loss of heterozygos ity,LOH)与乳腺癌发生发展之间的相关性。方法:采用微切割技术和PCR-SSLP银染技术结合微卫星重复序列多态性,分析45例乳腺癌石蜡库存标本中3p25、3p22-24、3p21.3、3p21.2-21.3、3p14.3、3p14.2和3p12等多个位点的杂合性缺失(LOH)。结果:45例乳腺癌标本中的LOH检出率分别为3p25(48%)、3p22-24(61%)、3p21.3(69%)、3p21.2-21.3(58%)、3p14.3(41%)、3p14.2(45%)和3p12(35%),其中3p21.3区域LOH出现率最高(69%)。分析各3p位点LOH与临床分期、淋巴结转移和雌孕激素受体(ER、PR)的相关关系,发现3p25区的LOH与孕激素受体表达的缺失之间具有相关性(P=0.006);3p22-24区的LOH与乳腺癌临床分期之间具有相关性(P=0.009)。结论:通过联合检测染色体3p区域多个位点的LOH将有助于乳腺癌的诊断和判断患者的预后。
Objective：To investigate the frequency of loss of heterozygosity （LOH） at several 3p regions in breast cancer tissues. Methods：Forty-five archival, paraffin-embedded,surgically resected breast cancer specimens were studied. Precisely identified malignant and normal lymph node tissues were microdissected from stained slides and analyzed for allele loss using polymerase chain reaction-based assays for dinucleotide repeat polymorphisms at six chromosome 3p loci （3p25,3p22-24,3p21.3,3p21.2-21.3,3p14. 3,3p14. 2, and 3p12）. Results：The 3p21.3 region had the highest frequency of LOH（67%）, followed by 3p22-24（61%）,3p21.2-21.3（58%） ,3p25（48%）, 3p14. 2（45%） ,3p14. 3（41%）, and 3p12 （35%）. There was a statistically significant correlation between loss of progesterone receptor expression and LOH at 3p25 （P=0. 006） and poor clinical stage and LOH at 3p22-24 （P=0. 009）. Conclusions：These findings suggest that the measurement of LOH at 3p may be an useful predicting marker for diagnosis and prognosis of patients with breast cancer.
出处
《中国误诊学杂志》
CAS
2007年第10期2184-2186,共3页
Chinese Journal of Misdiagnostics
基金
深圳市卫生科技计划攻关项目(编号:200405001
200204047)
关键词
乳腺肿瘤/遗传学
杂合子丢失
Breast neoplasms/genetics
Loss of heterozygosityheterozygosity
