摘要
含有大麦核糖体失活蛋白（ＢＲＩＰ） 编码序列的质粒ｐＲＣ２４／ＢＲＩＰ改造后， 依次与含有水稻碱性几丁质酶基因ＲＣＨ１０ 和水稻酸性几丁质酶基因ＲＡＣ２２ 编码序列的质粒ｐＡＢＣ２ 以及含有苜蓿β１ ， ３葡聚糖酶编码序列的质粒ｐＡＡＧ８９ 连接， 得到了中间载体ｐＲＡＳ１０ ．ｐＲＡＳ１０再与根癌农杆菌双元载体ｐＣＡＭＢＩＡ１３００ 连接， 得到了适合水稻转化的双元载体ｐＲＡＳ１３００ ． 利用冻融法将此表达载体导入根癌农杆菌ＬＢＡ４４０４ 中， 并准备将ｐＲＡＳ１３００ 转入水稻，
Mediate vector pRAS10 was obtained by ligating the modified plasmid pRC24/B RIP containing barley\|ribosome inactivating protein(B RIP) coding sequence with plasmid pABC2 containing rice basic chitinase gene RCH10 and rice acidic chitinase gene RAC22 coding sequence and then with plasmid pAAG89 containing alfalfa β 1,3 glucanase coding sequence pRAS10 was ligated to Agrobacterium tumefaciens binary vector to produce plant expression vector pRAS1300 which was suitable for rice transformation The expression vector was transformed into A tumefaciens LBA4404 using the Freeze shaw method The rice transformation using the constructed plant expression vector is in progress, in order to obtain rice varieties having broad and strong anti fungi traits.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
1999年第5期67-71,共5页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
国家863 高技术计划
关键词
真菌病
植物表达载体
构建
抗真菌病基因
fungi disease
chitinase gene
glucanase gene
ribosome inactivating protein gene
plant expression vector construction
