摘要
在内蒙古呼伦贝尔草原检获土耳其斯坦东毕吸虫幼虫期发育程度各不相同的阳性萝卜螺,通过组织学和组织化学方法对其幼虫期进行了观察. 参考周述龙(1991)对日本血吸虫尾蚴幼虫期进行的分期,把土耳其斯坦东毕吸虫的尾蚴发育过程也分为 5 期,尾蚴发育的胚细胞期、胚球期、尾蚴雏体期、尾蚴成熟前期和尾蚴成熟期. 子胞蚴育腔内胚细胞分裂,形成胚球,胚球中出现体细胞和胚细胞的分化,胚细胞结合疏松,胞浆中含酸性粘液和大量汞溴酚蓝反应阳性的碱性蛋白成分. 尾蚴成熟前后在三种腺体的呈色反应方面和日本血吸虫相似,其后钻腺腺体部分 P A S反应呈阳性的含糖类物质在雏体期后期开始分泌,逐渐由腺管通向头器末端. 两种尾蚴在尾蚴的体实质部分,对 P A S和汞溴酚蓝反应的呈色结果有着较大的差异. 另外,通过扫描电镜对土耳其斯坦东毕吸虫的尾蚴进行了比较观察,它们在头器的结构、体棘的形态以及感觉器的分布上存在着一定的差异.两种血吸虫的相似性表明在系统演化上有着密切的联系.
Histological study on each different development stages of larval trematode of Orientobilharzia turkestanica in Radix from Ewenk county, Inner Mongolia. According to Bi Xiao yun′s observation on Schistosoma japonicum, cercarial debelopment of O. turkestanica could also be divided into 5 stages: germinal cells stage, germball stage, embryo cercariae stage, premature cercariae stage and fully developed cercariae stage. Alcian PAS reaction and Mercury Bromphenol Blue (HgPBP) tests show that germinal cells in the germball are very rich in acid mucus and basic protein. The primitive penetration gland cells are established in this stage and the secrets turn out during later part of embryo cercarial stage. High simularity of O. turkestanica and S. japonicum suggest a close relationship existed between them. SEM observation on cercariae of O. turkestanica and S. japonicum also shows that they are very alike except the difference in the construction of head organ and morphology of the spine as well as the distribution of the sensory papillae.
出处
《厦门大学学报(自然科学版)》
CAS
CSCD
北大核心
1999年第5期769-775,共7页
Journal of Xiamen University:Natural Science
关键词
组织化学
扫描电镜
发育
血吸虫
幼虫期
宿主
Orientobilharzia turkestanica,Radix, Development, Histochemistry, Scanning electron microscope observation