摘要
目的:探讨Bmi-1在非小细胞肺癌(NSCLC)组织中的表达及其与临床病理特征的关系。方法:采用RT-PCR检测30例非小细胞肺癌及20例癌旁组织中Bmi-1mRNA的表达情况,同时应用免疫组织化学SP法检测52例非小细胞肺癌及30例癌旁组织中Bmi-1蛋白表达情况。结果:非小细胞肺癌组织中Bmi-1mRNA表达量明显高于癌旁组织(t=5.188,P<0.01)。肺癌组中Bmi-1蛋白的阳性表达率为67.3%(35/52),明显高于癌旁组13.3%(4/30),且表达量差别有高度统计学意义(Z=-4.837,P<0.01);肺癌组中Bmi-1蛋白阳性表达与癌组织的TNM分期及有无淋巴结转移有关(Z=-2.567,-2.366,P<0.05),而与患者的性别、年龄、组织类型、分化程度等无关(P均>0.05);结论:Bmi-1在非小细胞肺癌中的高表达与肿瘤发生发展及转移相关,可成为早期诊断肺癌及判断转移的重要参考指标。
Objective：To investigate the relationship between the expression of Bmi-1 in nonsmall-cell lung cancer（NSCLC） and clinicopathologic characters.Methods：Reverse transcription-PCR（RT-PCR）was applied to detect Bmi-1 mRNA in 30 of NSCLC speci-men and 20 of tumor-adjacent tissue.Immunohistochemistry was performed to detect the expression of Bmi-1 in 52 of NSCLC specimen and 30 of tumor-adjacent tissue.Results：The expression of Bmi-1mRNA in NSCLC specimen was remarkably higher than that in tu-mor-adjacent tissue（t=5.188,P0.01）.The positive rate of Bmi-1 expression was 67.3%（35/52）in NSCLC specimen and 13.3%（4/30） in tumor-adjacent tissue,respectively.There was a statistically significant difference for the expression of Bmi-1mRNA between NSCLC specimen and tumor-adjacent tissue（Z=-4.837,P0.01）.Bmi-1 expression in NSCLC was correlated with both TNM staging and lymph node metastasis（Z=-2.567,-2.366,P0.05）,but which had no relationship with sex,age,histotype and differentiation（P 0.05）.Conclusion：High expression of Bmi-1 in NSCLC is related with the development and metastasis of tumorigenesis.
出处
《现代生物医学进展》
CAS
2011年第7期1301-1304,共4页
Progress in Modern Biomedicine
